Culture of DII labeled hAECs on nanochitosan/PPy–Alg scaffold and Noggin-loaded nanochitosan/PPy–Alg scaffold In order to track hAECs in animal studies, we used 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DII). The sterilized scaffolds were incubated in a complete ...
Acknowledgements The authors acknowledge research grant from Bandung Institute of Technology through Riset Kolaborasi Indo- nesia 2024 program [contract no. 1841/IT1.B07.1/TA.00/2024; code file LPPM1.PN-6-32-2024]. The authors also acknowledge research grant from Padjajaran University ...
Chitosan coated TNTs: (e) thin coat of chitosan on indo-loaded TNTs (before release experiment), and (d) clear signs of chitosan degradation and visible open pores of TNTs (after completion of the drug release experiment). Indo: indomethacin, TNTs: titania nanotubes. The release of loaded...
Nanobody fusions for visualizing and manipulating intracellular signalling. Plasmids were generated that encode nanobodies specific for GFP variants (GNb) or RFP variants (RNb), fused to functional modules. Nanobody fusions with an N-terminal signal sequence to target them to the secretory pathway ar...
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The obtained cDNA was used to assess the hepatic expression of TGFβR1, TGFβR2, and COL3A1 genes by PCR using QuantiTect SYBR Green PCR Master Mix (Qiagen, Germany). The control GAPDH (Qiagen, Germany) was used as a reference normalizing gene for mRNAs quantification. The primer sets us...