Nano-Glo® Live Cell Assay System 简明操作指导 注意:这是节选操作步骤,详细英文说明书见产品N2011,N2012和N2013简要操作说明www.promega.com/protocols/ Nano-Glo®Live Cell Reagent是一种非细胞裂解性的检测试剂, 可用于监测活细胞中来自NanoLuc®萤光素酶或NanoBiT®检测...
The extended signal stability of the Nano-Glo® Dual-Luciferase® Reporter Assay enables an easy, add-mix-read assay protocol. Reagents can be used with injection-based protocols or with cell lysates made with passive lysis buffer. Homogeneous Assay Format:Assay...
The fluorescence of the released sEVs were continuously calculated using GloMax® Discover Microplate Reader (Promega, Madison, Wisconsin, USA). Representative data demonstrates at least three independent experiments (N ≥ 3). 4.20. Biodegradation assay of hydrogel Hydrogels were implanted subcutaneously...
24 h after transfection, cells were seeded into black, clear-bottom 384-well plates (20,000 cells per well) in assay media (FluoroBrite DMEM and the NanoLuc substrate NanoGlo (Promega, catalog no. N2012)), and incubated for 45 min at 37 °C. Cells were pre-incubated with ...
The continued emergence and zoonotic threat posed by coronaviruses highlight the urgent need for effective antiviral strategies with broad reactivity to counter new emerging strains. Nanobodies (or single-domain antibodies) are promising alternatives to
The plate was incubated in the dark for 2 min then read on the GloMax Multi+ Detection System (Promega). To quantify GFP expression, cells were plated in clear flat-bottomed 96-well plates (Nunc) and imaged every hour using the IncuCyte S3 live cell imaging system (Essen BioScience)....
the collected cell culture supernatants were loaded into 96-well plates containing 50,000 TZM-bl cells per well in RPMI 1640 medium with 10% FBS, and incubated at 37 °C for 48 h. After washing, the incubated TZM-bl cells were measured by Beta-Glo assay kit (Promega, Madison, WI...
Drug solutions used for the Tango assay were exactly the same as used for the cAMP assay. The next day, media and drug solutions were removed and 20 µl per well of BrightGlo reagent (purchased from Promega, after 1:20 dilution) was added. The plate was incubated for 20 min at room...
Luciferase activity was measured using Promega's luciferase assay system on GLOMAX 96 microplate luminometer (Promega, Sweden). For control experiments, AF-594-conjugated transferrin, AF-555-labeled cholera toxin B subunit (CtxB) and 70 kDa TAMRA-dextran (all from Molecular Probes) were applied ...
(20 µl per well) for overnight incubation. Drug solutions used for the Tango assay were exactly the same as used for the cAMP assay. The next day, media and drug solutions were removed and 20 µl per well of BrightGlo reagent (purchased from Promega, after 1:20 dilution) was...