Recently, we developed a nanobody-based single-cell CUT&Tag (nano-CT) protocol to simultaneously profile three epigenetic modalities—two histone marks and open chromatin state—from the same single cell. Nano-CT implements a new set of secondary nanobody-Tn5 fusion proteins to direct barcoded ...
F.T. conceived and supervised the project. F.T. and Q.L. designed the experiments. Y.G. designed the bioinformatic pipelines and conducted the data analysis with help from Z.J.; Q.L. developed the scNanoSeq-CUT&Tag protocol for the Nanopore platform; Q.L. and Z.W. performed the ex...
Probing histone modifications at a single-cell level in thousands of cells has been enabled by technologies such as single-cell CUT&Tag. Here we describe nano-CUT&Tag (nano-CT), which allows simultaneous mapping of up to three epigenomic modaliti
Eluted fractions were collected, analyzed by SDS-PAGE, and concentrated using Amicon concentrators with the appropriate molecular weight cut-off (Millipore, Bedford, MA). Mt-DyP and Mt-BfrB were further purified on a Superdex 200 HiLoad 16/60 gel filtration chromatography column (GE Healthcare)...
Setting a log2 fold change (log2FC) cut-off at |log2FC| ≥ 1 reduced this list to 643 significantly altered protein-coding genes (344 up- and 299 down-regulated). Performing gene set enrichment analysis (GSEA) allowed us to identify pathways associated with TGF-ß stimulation in SAS ...
Furthermore, a solution will be devised and its effectiveness will be stated that will pave its way in the future activities of energy efficient nano-routing protocols.doi:10.1007/978-3-030-78459-1_3Iqra IqbalAnca D JurcutMuhammad Mohsin Nazir...
MQTT broker is a shortcut to solve the reusable requirement of data stream, and NanoMQ, as a message service mainly for edge computing scenarios, multi-cloud bridging is also a function commonly needed by users. Before v0.9.0, NanoMQ's MQTT bridge only supported a single target. The ...
Briefly, the aortic arc identified by the appearance of aortic valve leaflets was cut and immersion-fixed in 10% buffered formalin. The formalin-preserved tissue was gradually dehydrated, embedded in paraffin, and serially cut into sections with 5 μm thickness and intervals of 50 μm. ...
Because nano-Tn5 binds directly to the primary antibodies, secondary antibody incubation from the CUT&Tag protocol is not required and is thus omitted (Fig. 1c). The monovalent interaction of the nanobody with the primary antibody further allows to combine the antibody and nano-Tn5 incubation ...
Resected breast tissues were immediately placed in cold, sterile DMEM after excision and cut into 2–4 mm pieces before undergoing enzymatic and mechanical dissociation using the gentleMACS Octo Dissociator multitissue kit and protocol (Miltenyi Biotec). Resuspended cells were then promptly plated ...