We target here the development of a biosensor suitable to determine the cellular NADP(H) redox status. For this, we combine two molecular modules: a rice NADPH-dependent thioredoxin reductase C (NTRC) and roGFP2 as NADPH sensing and output components, respectively. We thus exploit the ability ...
The lack of a deuterium isotope effect in the reduction of thio-NADP+ by NADH at low pH is also consistent with the view that NADPH release from the enzyme is slow under these conditions. A steady-state rate equation is derived for the reduction of AcPdAD+ by NADPH plus NADH, assuming ...
This result is in contrast to the glycohydrolase SPN of S. pyogenes, which is 20-fold less efficient at hydrolyzing NADP+ compared with NAD+ (13). However, the observation that the reduced nucleotides, NADH and NADPH, are not substrates of TNT is similar to other glycohydrolases such as ...
The volume inside the reactor was varied from 100 mL up to 400 mL by adding the glucose solution accordingto the equation: V_(ad) = 0.15.t – 0.01875.t2 [V_(ad) = added volume up to a given time (t)].Fadi Antoine Taraboulsi-Jr...
Turnover rates are expressed as the number of NADPH molecules consumed by one homodimeric FNR. The Michaelis constant (KM) and turn over number (kcat) were estimated by nonlinear regression analysis using the Michaelis-Menten equation in Igor Pro software (ver. 6.3, WaveMetrics, Portland, OR, ...
The kinetics for the NADPH inhibition suggested that n > 1 in the rate equation v = V[NADPH](Km + [NADPH]+ [NADPH]n+1Ki); i.e., more than two NADPH molecules bind to enzyme. The Km for 16-oxohexadecanoate did not change from pH 7.5 to 9.0 but increased about 10-fold from pH...
Our work led to the finding of a different, oxidative deformylation of 10-CHO-H 4 PteGlu according to equation 2. 10 CHO H 4 PteGlu + NADP + + H 2 O → H 4 PteGlu + CO 2 + NADPH + H + (2) This communication describes the partial purification of this new enzyme activity ...
are normally present as monomeric proteins in plastids, bacteria, mitochondria, and apicoplasts of intracellular parasites, where they catalyze the reaction described in equation (1), using noncovalently bound FAD as a prosthetic group.2 Fd(Fe+2) + NADP+ + H+⇆2 Fd(Fe+3) + NADPH ...