1.1. NAD+ and NADP+dependent isocitrate dehydrogenase (ICDH) activities in homogenates of Crassostrea virginica Gmelin adductor muscle have been separated.2.2. Investigation of the kinetic properties of oyster NAD+-dependent ICDH reveals that the Km(IC) is 1.58 mM in the absence of ADP and falls...
Lancien, M., Gadal, P., Hodges, M., Molecular characterization of higher plant NAD-dependent isocitrate dehydrogenase: evi dence for a heteromeric structure by the complementation of yeast mu- tants, Plant J., 1998, 16(3): 325 353.
OPEN SUBJECT AREAS: METABOLOMICS ENVIRONMENTAL SCIENCES Received 17 February 2014 Accepted 15 July 2014 Published 5 August 2014 Correspondence and requests for materials should be addressed to Y.Ka (kanda@nihs.go. jp) NAD-dependent isocitrate dehydrogenase as a novel target of tributyltin in human ...
Pig heart NAD-dependent isocitrate dehydrogenase requires NAD as coenzyme but binds NADPH, as well as NADH, ADP, and ATP, at regulatory sites. Incubation of 1–3 m m oNAD or oNADH with this isocitrate dehydrogenase causes a time-dependent decrease in activity to a limiting value 40% that ...
www.nature.com/scientificreports OPEN Molecular mechanism of the allosteric regulation of the αγ heterodimer of human received: 18 October 2016 accepted: 13 December 2016 Published: 18 January 2017 NAD-dependent isocitrate dehydrogenase Tengfei Ma1,Yingjie Peng1, Wei Huang1 & Jianping ...
NAD(+)-dependent isocitrate dehydrogenase from pig heart is an allosteric enzyme that is activated by ADP and is inhibited by NADPH in the presence of NADH. Transferred nuclear Overhauser effect measurements, made at a range of times to ensure that observed effects are due to direct dipole-dipol...
Yingjie Peng1, Wei Huang1,Yabing Liu2 & Jianping Ding1,3,4,5 Human NAD-dependent isocitrate dehydrogenase existing as the α2βγ heterotetramer, catalyzes the decarboxylation of isocitrate into α-ketoglutarate in the Krebs cycle, and is allosterically regulated by citrate, ADP and...
Structure, functions and regulation of NAD and NADP dependent isocitrate dehydrogenases in higher plants and in other organisms The distribution, intracellular localization, molecular structure, kinetic and regulatory properties of NAD- and NADP- isocitrate dehydrogenase are reviewe... RD Chen,P Gadal ...
Molecular mechanism of the dual regulatory roles of ATP on the αγ heterodimer of human NAD-dependent isocitrate dehydrogenase Association of the malate dehydrogenase-citrate synthase metabolon is modulated by intermediates of the Krebs tricarboxylic acid cycle ...
Pig heart NAD-dependent isocitrate dehydrogenase is inactivated by adenosine 5‘-O-[S-(4-bromo-2,3-dioxobutyl)thiophosphate] (AMPS-BDB) with incorporation of 1.78 mol of reagent/mol of average subunit. Complete protection against the inactivation is provided by 20 mM isocitrate + 1 mM Mn2+,...