Promega Corporation Cat no: G9071 TheNAD/NADH-Glo™ Assayis a bioluminescent, homogeneous single-reagent-addition assay for detecting total oxidized and reduced nicotinamide adenine dinucleotides (NAD+ and NADH, respectively) and determining their ratio in biological samples or in defined enzyme reacti...
Our pursuit of a pharmacological approach to boost intracellular NAD+levels focuses on discovering compounds that increase the activity of NAMPT. High-throughput screening (HTS) of a small molecule library using a protein thermal shift (PTS) assay17yielded novel NAMPT ligands. Subsequent evaluation wit...
NAD+/NADH‑Glo™ bioluminescent assay Control or KO neurons were cultured in a 96-well plate at a density of 1.3*103 cells/mm2 (4.235*104 cells onto 32 mm2 surface). Following manufacturer's instruction (Promega), neuronal cultures at DIV8 were washed three times with PBS and ...
The supernatant was diluted 10-fold with ddH2O and used for NAD and NADP measurement with the NAD/NADH-Glo™ Assay and NADP/NADPH -Glo™ Assay kits (Promega, Cat#G9071 and G9081) according to the user’s manual. All reactions were prepared on ice. Values were normalized to the ...
Pre-weighed tail epidermis, dorsal epidermal scrapes or liver tissue were lysed according to manufacturer recommendations for the NAD+ detection kit (Promega NAD/NADH-Glo assay). Briefly, samples were homogenized in ice-cold base solution (0.2N NaOH (Fisher Scientific) +1% dodecyltrimethylammonium ...
NAD/NADH-Glo Detection Reagent Promega Cat# G9071 LIVE/DEAD BacLight Bacterial Viability Kits Thermo Fisher Scientific Cat# L7007 Zombie Aqua Fixable Viability Kit BioLegends Cat# 423101 RNeasy mini kit QIAGEN Cat# 74104 RNeasy Protect Bacteria Mini Kit QIAGEN Cat# 74524 Penta His HRP conjugate...
NAD+and NADH concentrations were determined using the bioluminescent NAD/NADH-Glo Assay from Promega (Madison, WI). Cells were plated at a density of 1.5–3 × 104cells per well in 250 μL complete media on 96-well plates. For determining the effects of treatments on NAD+/NADH rati...
本发明提供了一种基本上由葫芦巴碱组成或由葫芦巴碱组成的组合物。该组合物可用于食品或饮料应用、制药或用作膳食补充剂。该组合物可施用于哺乳动物,以在对其有需要或处于其风险中的个体中治疗或预防线粒体相关疾病或与改变的线粒体功能相关的病症。线粒体相关疾病或病症选自以下项:衰老的有害效应、应激(例如,氧...
使用生物发光测定(promeganad/nadh-glotm#g9071)测量nad。这一情况在图1a中示出。[0147]来自野生型斑马鱼的胚胎在标准实验室条件下于28℃下进行抚育,并且在受精后96小时之时在6孔板中进行抚育(n=20-25)。用葫芦巴碱(sigma#t5509)治疗幼体16小时。使用比色nad定量测定法(biovisionnad/nadhquantitationcolorimetric...
After 6 h, ATP and NAD(H) levels were measured by CellTiterGlo and NAD/NADH-Glo assays (Promega). Readings were normalized to cell numbers measured with Casy (OMNI Life Sciences) on a mirror plate. Data analysis and visualization Exploratory data analysis and visualizations were performed in...