The NADP/NADPH-Glo™ Assay is selective for NADP+ and NADPH Rapidly Assess Total, NAD(P)+ and NAD(P)H Changes Directly in Cells in Culture Wells Detergent present in the reagent lyses cells, allowing detection of total cellular NAD+ and NADH or NADP+ and NADPH. For...
The NAD/NADH-Glo™ Assay is a bioluminescent, homogeneous single-reagent-addition assay for detecting total oxidized and reduced nicotinamide adenine dinucleotides (NAD+ and NADH, respectively) and determining their ratio in biological samples or in defined enzyme reactions. Complete the Best Quote fo...
Discovery of SBI-797812, a small molecule NAMPT activator.aCompiled melting temperature (Tm) data for human NAMPT treated with chemical compounds (N = 57,004) and tested with the PTS assay protocol (green). NAMPT ligands produced an upward Tm shift. DMSO was the Neg Control (blue). C...
f, Cells with or without ectopic V5-tagged HMGA1 expression with or without NAMPT knockdown were examined for the NAD+ levels using the NAD+/NADH-Glo Assay. g, The gating strategy used for determining mean fluorescence intensity. h, In established senescent cells induced by oncogenic RAS, HMG...
4 Loss of NMNAT2 reduces NAD+ levels and impairs the NAD redox potential in distal axons. A Levels of N AD+ and NADH and calculated NAD+/NADH ratios in DIV8 ctrl and KO cortical neurons as measured by the NAD+/NADH-Glo assay and...
Activity was normalized to the protein content of the sample lysates used in the assay. 2.6. NAD+ measurement Pre-weighed tail epidermis, dorsal epidermal scrapes or liver tissue were lysed according to manufacturer recommendations for the NAD+ detection kit (Promega NAD/NADH-Glo assay). Briefly,...
ATP production assay UOK109 cells with different treatment were cultured in a 6-well plate (3 × 105 cells/per well) for 24 h. After treatment, the production of ATP in each group was detected by an Enhanced ATP Assay Kit and GloMax96 according to the manufacturer’s protocol. Cell...
NAD+and NADH concentrations were determined using the bioluminescent NAD/NADH-Glo Assay from Promega (Madison, WI). Cells were plated at a density of 1.5–3 × 104cells per well in 250 μL complete media on 96-well plates. For determining the effects of treatments on NAD+/NADH rati...
Total cellular NAD+ content was determined using the NAD/NADH-Glo® Luminescence Assay (#G9071, Promega) according to the manufacturer’s protocol and quantified as described for the ATP measurement. Reactive oxygen species measurement The levels of cellular reactive oxygen species (ROS) were ...
Filtered AWF was immediately used for NAD and NADP measurement with the NAD/NADH-Glo™ Assay and NADP/NADPH -Glo™ Assay kits according to the user’s manual. AWF was kept on ice and all reactions were prepared on ice. Values were normalized to the leaf weight. 13C-NAD+ treatment ...