About this Antibody Name N6-Methyladenosine (m6A) Rabbit Polyclonal Antibody Category Rabbit Polyclonal Antibody (pAb); Primary antibody Isotype IgG Purification method Affinity purification Positive samples Arabidopsis Cellular location - Customer validation - About the Immunogen Immunogen Chemical...
N6-Methyladenosine (m6A) Rabbit Polyclonal Antibody -20℃保存,Western一抗稀释液-20℃或4℃保存,一年有效。Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。 注意事项: 如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,...
For antibody-based applications requiring high specificity to N6-methyladenosine (m6A)Citations (17) | (1) | Write a Review Application: DB, ELISA, IF, IP, MeRIP Areas of Research: RNA Methylation Clonality: Polyclonal Conjugate: Unconjugated Host: Rabbit Isotype: IgG Purification: Affinity ...
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Fragmented RNA was incubated with an anti-m6A polyclonal antibody (Synaptic Systems, 202003) in immunoprecipitation (IPP) buffer for 2 h at 4 °C25. The reaction mixture was then immunoprecipitated with protein A magnetic beads (Thermo Fisher, MA, USA) at 4 °C for 2 h. Next, the bound ...
The antibody specifically recognized the oligos with m6A, while exhibiting low cross-reactivity to the oligos with only unmodified adenosine or containing m1A. b, m6A signal intensity in the immunoblot, measured by Image J, in the whole lane containing the poly(A)-enriched RNA of bloodstream ...
The membranes were incubated with rabbit polyclonal anti-SRD5A3 antibody (PA5-55480, Invitrogen, Carlsbad, CA, USA), mouse monoclonal anti-IGF2BP3 antibody (MA5-27480, Invitrogen), and mouse monoclonal anti-GAPDH antibody (MA5-15738, Invitrogen), followed by rinse with TBST and incubation ...
m6A Polyclonal rabbit purified antibody Synaptic Systems Cat# 202 003; RRID: AB_2279214 Anti-FLAG® M2 Magnetic Beads Sigma-Aldrich Cat# M8823; RRID: AB_2637089 Brilliant Violet 711 anti-mouse CD8a Antibody (clone 53-6.7) Biolegend Cat# 100747; RRID: AB_11219594 Brilliant Violet 785 anti-...
RNA samples were fragmented into 100 bp using fragmentation buffer and then incubated with anti-m6A polyclonal antibody (Synaptic Systems, 202,003, Germany) in immunoprecipitation (IP) buffer for 2 h at 4 °C. The mixture was then immunoprecipitated by incubation with protein-A beads (The...