Mechanism of N10-formyltetrahydrofolate synthetase derived from complexes with intermediates and inhibitors. Protein Sci. 21:219-228.Celeste LR, Chai G, Bielak M, Minor W, Lovelace LL, Lebioda L (2012) Mechanism of N10-formyltetrahydrofolate synthetase derived from complexes with intermediates and...
Formyltetrahydrofolate synthetase (FTHFS) from the thermophilic homoacetogen, Moorella thermoacetica, has an optimum temperature for activity of 55−60 °C and requires monovalent cations for both optimal activity and stabilization of tetrameric structure at higher temperatures. The crystal structures of...
ChemInform Abstract: Synthesis and Activity of a Tetrahydrofolate Inhibitor (I) of the Enzyme N10-Formyl-H4-folate-Met-tRNAfMet Transformylase.nucleic acidsA series of adenine nucleoside derivatives, most of them prepared for the first time, have been evaluated as substrates or inhibitors of ...
N 10 -formyltetrahydrofolic deacylase catalyzes the hydrolysis of N 10 -formyltetrahydrofolate into formate and tetrahydrofolate. It is found that a catalytic amount of TPN or TPNH is required in the deacylase reaction. It is observed that in the presence of a limiting amount of tetrahydro...
One-carbon metabolism produces methionine and N-formyl-tetrahydrofolate (N-fTHF) required for aminoacylation and formylation of initiator tRNA (i-tRNA), respectively. In Escherichia coli, N-fTHF is made from 5, 10-methylene-THF by a two-step reaction using 5,10-methylene-THF dehydrogenase/...
The catalytic mechanism of one-carbon unit transfer between 5-amino-4-carboxamide imidazole (AICA) and N10-formyl-tetrahydrofolate (10f-H4F) is investigated by ONIOM approach. There are two different channels for proton transfer in this reaction, resulting in two reaction pathways with different ...
H. (1994) Conforma- tion of ATP and ADP bound to N10-formyltetrahydrofolate synthetase determined by TRNOE NMR spectroscopy, Biochemistry 33, 693Ϫ 698.Song, S. , Velde, D. V. , Gunn, C. W. & Himes, R. H. ( 1994 ) Conformation of ATP and ADP bound to N 10 -formyltetrahydro...
The crystal structure of N10- formyltetrahydrofolate synthetase 1 (FTHFS 1) and N10- formyltetrahydrofolate synthetase 2 (FTHFS 2) from the syntrophic acetate oxidizing bacterium, Tepidanaerobacter acetatoxydans Re1, was solved by x-ray crystallography to a resolution of 2.15 Å, and 2.30 ...
R. HKounga, K., Song, S., Haslam, G. C. & Himes, R. H. (1996). Site-directed mutagenesis of putative cataly- tic and nucleotide binding sites in N10-formyltetra- hydrofolate synthetase. Biochim. Biophys. Acta, 1296, 112-120....
Formyltetrahydrofolate synthetase (FTHFS) from the thermophilic homoacetogen, Moorella thermoacetica, has an optimum temperature for activity of 55-60 degrees C and requires monovalent cations for both optimal activity and stabilization of tetrameric structure at higher temperatures. The crystal structures...