While a recent report indicates that LQT in some patients is associated with a mutation of HERG at a consensus extracellular N-linked glycosylation site (N629), earlier studies failed to identify a role for N-linked glycosylation in the functional expression of voltage-gated K + channels. In ...
To investigate the effects of IgG-BCR Fc glycans on B-cell function, we made use of the Burkitt lymphoma B-cell line (Ramos) and knocked out the N(297)-linked glycosylation site on mIgG. Ramos cells are human GC-derived B cells that express co-receptors in close proximity to the BCR ...
N-linked glycositeSkylinePopulus trichocarpaMonolignolN-linked glycansOur greater understanding of the importance of N-linked glycosylation in biological systems has spawned the field of glycomics and development of analytical tools to address the many challenges regarding our ability to characterize and ...
Secreted phospholipase B enzymes (PLB1) with high levels of N-linked glycosylation are proven fungal virulence determinants. We demonstrated that removal of N-linked glycans from secreted cryptococcal PLB1 leads to loss of enzyme activity. To determine if individual N-glycan attachment sites affect ...
In addition, insights into the location and residues in and around the acceptor tripeptide sequon suggest an influence on N-glycan site occupancy. Insights from these characterizations are being used to elucidate methodologies to control N-glycosylation site heterogeneity.Jullian Jones and Sharon S. ...
The second stage uses an SVM trained on glycoproteins only to predict for each N-X-S/T sequon in the query protein whether it is a glycosylation site. N-GlyDE then integrates the results of the first-stage prediction for weight adjustment of the second-stage prediction results to yield the...
•UDP-Glc,UDP-Gal•UDP-GlcNAc,UDP-GalNAc•UDP-GlcA,UDP-Xyl•GDP-Man,GDP-Fuc •CMP-Sia(CMP-Neu5Ac)2.Givetherepresentationofthefollowingoligosaccharide:Chapter2 N-linkedGlycosylation AlleukaryoticcellsproduceN-glycans.N-linkedglycosylationisthebestunderstoodroutetoproteinglycosylation.Themolecular...
glycosylation is one of the most abundant and diverse forms, and it is common in all eukaryotic cells [27,28]. Interestingly, increasing evidence has shown that both PD-L1 on tumor cells and PD-1 on tumor-specific T cells undergo extensive N-linked glycosylation and that this modification pl...
HIV-1 Env gp120 C2V5 Potential N-Linked Glycosylation site(s) (PNGs) and amino acid length polymorphisms among infected family members. Advances in Infectious Diseases, 2011,1:1-13 doi:10.4236/aid.2011.11001ChandiwanaDuri, Kerinaduri... K Chandiwanaduri - 《Duri》 被引量: 0发表: 2013年 ...
We predicted that the N-glycosylation site Asn152, which is located at the middle of the Ig-V domain on CD147, would disturb the interactions between CD147 and the partner proteins that assist in protein folding, traf- ficking, and/or expression22,24. In screening the CD147(N152Q)-...