Overall, flavin-dependent N-hydroxylating enzymes can accept amines, diamines, amino acids, amino sugars, and amino aromatic compounds and thus provide access to versatile families of compounds containing the N-O motif. Natural roles as well as synthetic applications are highlighted.Key points N-O...
Two structures of an N-hydroxylating flavoprotein monooxygenase: ornithine hydroxylase from Pseudomonas aeruginosa. The ornithine hydroxylase from Pseudomonas aeruginosa (PvdA) catalyzes the FAD-dependent hydroxylation of the side chain amine of ornithine, which is subse... J Olucha,KM Meneely,AS Chilto...
Mechanistic and structural studies of the N-hydroxylating flavoprotein monooxygenases. Bioorg Chem 2011;39:171-7.J. Olucha, A.L. Lamb, Mechanistic and structural studies of the N-hydroxylating flavoprotein monooxygenases, Bioorg. Chem. 39 (2011) 171-177....
Hydroxamate-containing siderophores are synthesized via the hydroxylation of the amino groups on the side chains of lysine and ornithine by flavin-dependent N-hydroxylating enzymes. The N6-lysine hydroxylase from Mycobacterium smegmatis (MbsG) and the N5-ornithine hydroxylase from Aspergillus fumigatus (...
The first two structures of a class B N-hydroxylating monooxygenase were determined with FAD in oxidized (1.9 脜 resolution) and reduced (3.03 脜 resolution) states. PvdA has the two expected Rossmann-like dinucleotide-binding domains for FAD and NADPH and also a substrate-binding domain, with...
L. (2011) Two structures of an N-hydroxylating flavoprotein monooxgenase: the orni- thine hydroxylase from Pseudomonas aeruginosa. J. Biol. Chem. 286, 31789 -31798Olucha, J., Meneely, K. M., Chilton, A. S., and Lamb, A. L. (2011). Two struc- tures of an N-hydroxylating ...
The first part of the thesis describes mechanistic studies of a member of a novel subclass of N-hydroxylating flavin-dependent monooxygenases. The enzyme, L-ornithine monooxygenase (OMO), activates oxygen to a reactive flavin hydroperoxide intermediate to hydroxylate the primary amine of L-ornithine...
2014. Structure of the quinoline N -hydroxylating cytochrome P450 RauA, an essential enzyme that confers antibiotic activity on aurachin alkaloids . FEBS Lett. 588 :105–110. 10.1016/j.febslet.2013.11.016 [ PubMed ] [ Cross Ref ]Yasutake Y, Kitagawa W, Hata M, Nishioka T, Oza- ki T...
Dual role of NADP(H) in the reaction of a flavin dependent N-hydroxylating monooxygenase. Biochim Biophys Acta 2012;1824:850-7.Romero, E., Fedkenheuer, M., Chocklett, S. W., Qi, J., Oppenheimer, M., and Sobrado, P. (2012) Dual role of NADP(H) in the reaction of a flavin ...
L. (2011) Mechanistic and structural studies of the N-hydroxylating flavoprotein monooxygenases. Bioorg. Chem. 39, 171-177.Olucha, J., and Lamb, A. L. (2011) Mechanistic and structural studies of the N-hydroxylating flavoprotein monooxygenases. Bioorg. Chem. 39, 171-177...