HYPK promotes the activity of the N-alpha-acetyltransferase A complex to determine proteostasis of nonAc-X-2/N-degron-containing proteinsTERMINAL ACETYLATIONNATAHUNTINGTINIDENTIFICATIONINTERACTSRIBOSOMEPLATFORMREVEALSSYSTEMDOMAINIn humans, the Huntingtin yeast partner K (HYPK) binds to the ribosome-associated...
NAT10 is a nucleolar protein that contains an acetyltransferase domain and a tRNA binding domain. NAT10 has histone acetylation activity and participates in the regulation of human telomerase reverse transcriptase. It is also involved in the DNA damage response and regulates cytokinesis [11,12]. NAT...
NLR family pyrin domain containing Nrf nuclear factor erythroid 2-related factor oxidative phosphorylation ONOO− peroxynitrite oxLDL oxidized low-density lipoprotein poly(ADP-ribose) polymerase pyruvate dehydrogenase complex PER periods PGC peroxisome proliferator-activated receptor gamma coactivator SIR...
Protein modifications are essential to modulate cellular protein activity, stability, subcellular localization, and interactions1. One of the most important protein modifications is acetylation, which can occur co- or post-translationally at the ε-amino group of lysine residues or the free α-amino g...
The gcn5 revertant and gcn5 mutant carrying the empty vector or chimeric construct were grown overnight in YEP containing 100 mM GlcNAc or glucose at 30 °C to saturation for Western analysis. The construct is schematically illustrated on the left (ADA2, ADA2 interaction domain; BrD, ...
f Structure (top) and functional domain (bottom) of human NAT10 protein. The different colors indicated distinct domain of NAT10. g RACK1 were isolated by immunoprecipitation (IP) and subjected to IB assays using FLAG (top panels) antibodies to evaluate the interaction between RACK1 and NAT10...
, this modifies the charges of the side chain of the corresponding domain, thus affecting the catalytic activity and protein level due to a reduction of protein thermostability. For both of these SNPS, the effect of protein level reduction is due to structural changes in the protein, not to ...
This subdomain contains a short helix (helix α3) whose C terminus is directed toward the interior of the protein and the active site (discussed below). The convergence of the three loops in AANAT creates a deep funnel leading into the interior of the protein (Figure 5A and Figure 5B). ...
The single domain α/β protein resembles a cupped right hand wrapped around a cylinder and consists of a highly curved, six-stranded β sheet of mixed polarity that is sandwiched between four α helices. The structure includes all four conserved GNAT motifs (C, D, A, and B) and ...
N-terminal acetylation (Nα-acetylation) is part of N-terminal protein processing events in a cellular context. The enzymes instrumental in acetylation of a diverse range of cellular proteins are known as Nα- acetyltransferases (NATs). Using an activated acetyl coA donor, NATs catalyze ...