糖苷内切酶S(EndoS)是一种细菌内源性β-N-乙酰氨基葡萄糖苷酶催化水解前两个-乙酰氨基葡萄糖之间的β-1,4键IgG Fc区域的双触角络合型N-链聚糖残基。可用于从糖蛋白中去除N-糖链。实验示例：1．用去离子水溶解1-20 ug的糖蛋白，加入1ul的10×GlycoBuffer 1，用去离子水定容到10 ul;2．加入1-2ul的Endo...
Endo-β-N-acetylglucosaminidase（Endo-S2）是一种内切N-乙酰葡萄糖胺酶，它是来自化脓性链球菌(streptococcus pyogenes)，能够切除糖蛋白N-连接糖链最内侧两个N-乙酰葡萄糖胺之间的糖苷键。对高甘露糖型、杂合型和双天线复杂型都有效果。酶学性质：来源：微生物 分类：EC 3.2.1.96 分子量：90 kDa (SDS-PAGE...
In pursuit of novel drug targets for the human pathogen, we studied peptidoglycan N-acetylglucosaminidases, whose structures are composed of two domains forming a V-shaped active site cleft. Combined insights from crystal structures supported by site-directed mutagenesis, modeling, and molecular ...
Endo-尾-N-acetylglucosaminidase (ENGase) catalyzes hydrolysis ofN-linked oligosaccharides. Although many ENGases have been characterized from various organisms, so far no fucose-containing oligosaccharides-specific ENGase has been identified in any organism. Here, we screened soil samples, using dansyl ...
Chemoenzymatic synthesis using engineered endo-β-N-acetylglucosaminidases (ENGases) has been used extensively to modulate the chemistry of N-glycosylated proteins. However, defining the molecular mechanisms by which ENGases specifically recognize and process N-glycans remains a major challenge. Here we...
Endo-β-N-acetylglucosaminidase(Endo A)文名是糖苷内切酶A，基因来源原菌节杆菌(Arthrobacter protophormiae)，是一种高度特异性糖苷内切酶，从糖蛋白中释放N-连接低聚糖，能识别高甘露糖型糖链。酶学性质 来源：微生物 分类：EC 3.2.1.96 分子量：67 kDa (SDS-PAGE)最适pH：8.0 最适温度：37°℃ 底物...
Bacterial endo-β-N-acetylglucosaminidases (EC 3.2.1.96; endoglycosidases) are widespread enzymes that cleave the N,N′-diacetyl chitobiose moiety characteristic of the pentasaccharide Man3GlcNAc2 found in all N-glycans (22). These enzymes belong to glycosyl hydrolase families GH18 or GH85. ...
作者: S.,E.,Winn,H.,R.,Ball 摘要: The pH, solids and protein content and β-N-acetylglucosaminidase activity of the four albumen layers and the shell and vitelline membranes were measured over a nine-day aging period on eggs stored at 25°C. The initial enzyme activity gradient observed...
A potent inhibitor of β- N -acetylglucosaminidases: 6-acetamido-6-deoxycastanospermine. Tetrahedron Lett. 1991; 32 :719–720.Liu PS, Kang MS, Sunkara PS. A potent inhibitor of beta-N-acetylglucosaminidases: 6-acetamido-6-deoxycastanospermine. Tetrahedron Lett. 1991; 32 :719–720....
β-N-Acetylglucosaminidase Activity of the Albumen Layers and Membranes of the Chicken's Egg - ScienceDirect SE Winn,HR Ball - 《Poultry Science》 被引量: 0发表: 1975年 Effect of cadmium injected in ovo on hatching results and the activity of plasma hydrolytic enzymes in newly hatched chicks...