Metabolic engineering facilitates chemical biosynthesis by rewiring cellular resources to produce target compounds. However, an imbalance between cell growth and bioproduction often reduces production efficiency. Genetic code expansion (GCE)-based orthogonal translation systems incorporating non-canonical amino aci...
Escherichia coli was modified by metabolic engineering to develop a fermentation process. Over-expression of glucosamine synthase (GlmS) and inactivation of catabolic genes increased glucosamine production by 15 fold, reaching 60 mg l −1. Since GlmS is strongly inhibited by glucosamine-6-P, GlmS ...
Figure 9.1.Major metabolic pathways associated with GlcNAc synthesis in the engineered cells. GlcNAc can be used as carbon source and enter EMP by multiple enzymatic steps including phosphorylation (from N-acetylglucosamine to N-acetylglucosamine-6-phosphate) by PTS system-N-acetylglucosamine-specific tra...
Interestingly, Rep1 is also required for galactose utilization, and GlcNAc can induce the expression of galactose metabolic genes in C. albicans26. The galactose signalling pathway is rewired in C. albicans and is not regulated by the Gal3, Gal80 and Gal4 regulatory circuitry59. In fact, the...
Here we review the applications, commercial market, and production of GlcN and GlcNAc, with emphasis on the metabolic and process engineering strategies used to improve GlcN and GlcNAc production by recombinant microbes.Long LiuKey Laboratory of Carbohydrate Chemistry and Biotechnology...
- 《Metabolic Engineering》 被引量: 24发表: 2013年 Production of N-Acetylglucosamine Using Recombinant Chitinolytic Enzymes The pharmaceutically important compound N-acetylglucosamine (NAG), is used in various therapeutic formulations, skin care products and dietary supplements... S Kumar,R Sharma,R ...
Thus, the synthesis of UDP-GlcNAc is critically dependent on the coordinated action of several key metabolic pathways in addition to glucose. The importance of the HBP and UDP-GlcNAc synthesis for cell viability is also emphasized by the fact that gene deletion of either EMeg32 or Ag...
However, the fact that overflow of metabolic by-products acetoin and acetate had been blocked by mutations in alsRSD and ackA, meant that pyruvate accumulated as an overflow metabolite in this strain (Fig. 1) [5, 6]. Though overexpression of CeGNA1 and EcGlmS decreased pyruvate accumulation ...
Enzymatic activities of HATs are regulated by phosphorylation, interaction with other proteins10, and the metabolic state of the cell12. However, the link between metabolic substrates and their ability to directly influence epigenetic signalling remains incompletely understood. As a part of the commensal...
O-GlcNAc signaling: a metabolic link between diabetes and cancer? Trends Biochem. Sci, 35 (2010), pp. 547-555 Google Scholar 7 C. Slawson, N.E. Zachara, K. Vosseller, W.D. Cheung, M.D. Lane, G.W. Hart Perturbations in O-linked β-N-acetylglucosamine protein modification cause sev...