SIGMA试剂,部分现货,周一,五定货,3周左右到货。 Millipore试剂,部分现货 耗材,每周三定货,2-3周到货。 Invitrogen试剂,部分现货,周三定货,2-3周到货。 ABCAM试剂,周三定货,2-3周到货。 产品规格: GR:优级纯。主成份含量很高、纯度很高,产品仅用于科研适用于精确分析和研究工作,有的可作为基准物质. AR:分析纯...
Acridine orange staining Acidic vesicles were quantified using acridine orange (AO) (Sigma-Aldrich, 318337); a pH sensitive dye used to detect lysosomal activity. We used flow cytometry or fluorescent microscopy to detect FL1/orange-red vs. FL3/green fluorescence. Cyto-ID staining Pre-autophagosom...
耗材:细胞培养耗材、普通实验耗材。 生化试剂:*生化试剂、进口分装生化试剂、国产生化试剂。 抗体:免疫组化、免疫荧光、流式细胞检测、免疫细胞化学。 公司代理品牌:RB .RND. Amresco 、prospec 、lifespan 、ABR、s 、spring、 abcam 、Santa Cruz、usbio BD耗材、Gibco试剂、 corning耗材 sigma试剂 ...
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were represented in each experimental condition using appropriate randomiza- homogenates were prepared in Tris-lysis buffer containing EDTA, EGTA, glycerol, triton-X100, sodium fluoride, sodium pyrophosphate, Na3VO4, DTT and a protease inhibitor cocktail (Sigma, Castle Hill, NSW, Australia; #p8340...
酰胺、甲叉双丙烯酰胺购自美国Sigma公司,去离 子甲酰胺购自上海生物化学试剂工程公司,其他 试剂均为国产分析纯。 紫外可见分光光度计购自尤尼科上海仪器有 限公司,生物冷冻离心机及普通PCR仪购自德国 Eppendoff公司,实时荧光定量PCR仪及DGGE电 泳系统购自美国Bio⁃Rad公司。 1.3 样品采集 饲养56d之后通过颈椎脱臼...
NAC was purchased from Sigma (St Louis, MO, USA). The dose of NAC used here was based on previous studies conducted in rats [7, 10]. Blood pressure was measured in conscious rats by an indirect tail-cuff method (BP-2000, Visitech Systems, Inc., Apex, NC, USA) at 4, 6, 8, 10...
The dogs in NAC group were injected intravenously with NAC (Batch No. 2009081606, Sigma, USA) (150 mg · kg-1) through femoral vein before CPB according to the method in reference [22]. Then the dogs were continuously infused with NAC (20 mg · kg-1 · h-1) during ...
ε-caprolactone were purchased from Sigma-Aldrich. Both globalide and ε-caprolactone (CL) were dried under vacuum (0.1 bar) and 100 °C, during 24 h [25] and also stored in a desiccator over silica and 4 Å molecular sieves. 2.2. Poly(globalide-co-ε-caprolactone) ...
Cells were washed twice with ice-cold phosphate buffered saline (PBS), fixed in 4% methanol-free formaldehyde in PBS for 25 Minutes at 4°C and permeabilized by 0.1% Triton X-100 (Sigma-Aldrich, MO, USA) in PBS for 5 Minutes (fixed cells incubated for 5 Minutes with DNase I buffer ...