However, when the NS mutant proteins and the N protein were synthesized individually and then mixed together, only the intact NS protein and NS mutant protein with 24 amino acids deleted from the C-terminus bound to the N protein, whereas deletion of 47 or more amino acids from the C-...
n-terminal hydrophobi n-terminus n acusticus n adamant bend n additional strength n annulus loss nbatesi ncinerea n euphotic zone nfossa n gonorrhoeae nindicao kuntze n magnetic inspection nmarina linn var gros nmeningitidis norientalis triest et npeltatum okuntze n phyllomanes ching n pilot ...
The genes for expression of the WW domain fragment fused with MBP at its C-terminus were inserted into a pET22b vector (NdeI/HindIII site). The linker sequences, which were Gly-Ser-Gly for WW11 and WW19 and Gly-Ser-Gly-Met for WW17, were inserted between the WW domain fragment and...
This property can be modified by the length of the NH2 terminus and the presence of splice inserts in the C-terminus of KCa1.1. Whether these structures are also involved in KCa1.1 functional association with the neuronal β4 subunit is unknown. The length of the NH2 terminus can vary as ...
Arabidopsis plants specifically recognize the N terminus of the protein, and an N-acetylated peptide comprising the first 18 amino acids, termed elf18, is fully active as inducer of defense responses. The shorter peptide, elf12, comprising the acetyl group and the first 12 N-terminal amino ...
A cDNA clone, with an insert of 3068 bp, encoding the N-terminus of human tripeptidyl peptidase II (TPP II) has been isolated and sequenced. Together with previous data (GenBank accession no J05299) a total of 4634 bp of the nucleotide sequence for TPP II can now be assembled. There...
As shown in Figure 2c, the expression of full-length p27 was able to completely abrogate induction of phospho-S6 by arsenite, whereas transfection of C terminus of p27 (aa 78–198) did not render the repressive effect, indicating that the N terminus of p27 (aa 1–51) was critical ...
Interactions between the Nun C terminus and RNA polymerase as well as the DNA template are required for termination. We have isolated Nun C-terminal point and deletion mutants that are unable to block transcription. The mutants bind NUT RNA and inhibit translation of the λ N gene. Thus HK...
MA Lety,C Frehel,P Berche,A Charbit 摘要: Summary A putative PEST sequence was recently identified close to the N-terminus of listeriolysin O (LLO), a major virulence factor secreted by the pathogenic Listeria monocytogenes . The deletion of this motif did not affect the secretion and ...
The C-terminal part (domain C) of the S. cerevisiae eRF3 is responsible for the function of the protein in translation termination. The N-terminal region of yeast eRF3 is non-essential for the translation termination and viability and may be further subdivided into the N-terminal (N) ...