Screening assays that allow for the identification of agents that modulate the activity of N-terminal acetylation of a polypeptide and the Doa10 branch of the N-end rule pathway are provided. Also provided are methods of using an agent that modulates the activity of N-terminal acetylation of a...
N-terminal acetylation:This is one of the most common modifications, where an acetyl group is added to the N-terminus of a peptide or protein. N-terminal alkylation:N-alkylated amide bonds can often change the intramolecular or intermolecular hydrogen bond interaction of peptides, thus affecting ...
N-terminal acetylation (Nt-acetylation) is a widespread protein modification among eukaryotes and prokaryotes alike. By appending an acetyl group to the N-terminal amino group, the charge, hydrophobicity, and size of the N-terminus is altered in an irrev
The N-terminal blocking of proteins occurs in a wide range of eukariotic cells, where more than 50% of the cytosolic proteins can be -αacetylated. The acetylation which occurs during or after the biosynthesis of the polypeptide chains serves to protect the intracellular proteins from proteolysis...
Investigation into putative human N-terminal acetyltransferases N-terminal acetyltransferases (NATs) acetylate the N伪 termini of polypeptide chains and N-terminal acetylation (Nt-acetylation) is one of the most abundant protein modifications in eukaryotic cells. Studies have found that approximately... ...
d (left) N-terminal acetylation inhibits SUMOylation. Lysates from CHO-K1 cells transfected with CFL1WT-HA, His-SUMO1, and Myc-Naa60, were subjected to De-IP and IB. (right) Quantification of Western blots. Mean ± SEM; ***p < 0.001, by two-tailed t test. e Diagram of...
Strikingly, proteins bearing N-terminal signal sequences are usually not N-acetylated [5]. If the modification is introduced by mutation, N-acetylation leads to missorting of secretory proteins to the cytosol. These observations led to the conclusion that N-acetylation interferes with ER targeting ...
Searches for peptides were performed with Mascot using the following parameters: a mass tolerance of 5 ppm on the precursor ion and 0.5 Da on the MS/MS, static modification of carboxamidomethylated Cys (+57.0215), and variable modifications of oxidized Met (+15.9949), N-terminal acetylation (...
Table I. N-terminal acetylation of the yeast 20 S proteasome subunits SubunitInternal sequenceN-terminal sequence of proproteinResults of Edman degradationNormal N-terminal sequence Normalnat1mak3nat3 α1 (Sclp1, C7) TDPAGYEV GYK MSGAAAASAAGYDRHITIFSPEGRLYQVE YAFKATNQTNI … Blocked SGAAAAS Block...
The enzymes instrumental in acetylation of a diverse range of cellular proteins are known as Nα- acetyltransferases (NATs). Using an activated acetyl coA donor, NATs catalyze an irreversible transfer of the acetyl moiety on to the free N-terminus of a nascent growing polypeptide, usually in...