N-terminal 6xHis-SUMO-tagged 产品提供形式: Liquid or Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your dema...
系统还具有在N端编码6xHis SUMO融合标签可以改善表达蛋白的可溶性。其他表达系统为鼠李糖启动子控制的表达。 5秒定向克隆PCR扩增基因 Expresso® T7 克隆和蛋白表达系统使用Expressioneering ™技术,是一个无酶的重组克隆策略到无缝基因载体整合过程。使用带有18bp载体互补序列的引物对靶向基因进行PCR扩增。不同于...
To test whether CFL1 is a SUMO substrate, we transiently transfected Chinese hamster ovary-K1 (CHO-K1) cells with the plasmid expressing C-terminal hemagglutinin (HA)-tagged mouse CFL1 (CFL1-HA), together with or without the plasmid for N-terminal His-tagged SUMO1 (His-SUMO1). Lysates ...
- Tightly-controlled expression of N- or C-terminal 6xHis tagged proteins. 产品应用 - The Expresso T7 Cloning and Protein Expression Systems are designed for fast, easy, and efficient directional cloning and expression of PCR-amplified genes. 组成成分 The Expresso T7 Cloning & Expression System...
N-terminal 6xHis-SUMO-tagged 产品提供形式: Liquid or Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your ...
(Millipore Sigma) using a 10 kDa membrane. Chemokine was diluted 4× with Refold Buffer and ULP1 added to cleave the N-terminal 6xHis-SUMO3-tag. The AKTA-Start system was used to load the cleaved protein onto a 5 mL column of SP Sepharose Fast Flow resin (GE Healthcare) equilibrated ...