After the resin was re-washed with 20 ml buffer A to reduce the ionic strength, CalA3 was eluted using 25 mL of elution buffer (25 mM HEPES pH 7.6, 300 mM imidazole pH 7.6, 50 mM NaCl, and 5% glycerol). The resulting elution was applied to a 5 ml HiTrap Heparin HP...
the C-7 oxygen atom of1was 2.8 Å from the imidazole ring of H27 and 2.8 Å from the carboxamide carbon of carbamoyl-AMP, indicating that H27 is inevitably protonated and subsequently initiates the reaction to drive deprotonated1to engage in nucleophilic attack at the carbamoyl group. ...
Ribosomally synthesized and post-translationally modified peptides (RiPPs) are a structurally diverse group of natural products. They feature a wide range of intriguing post-translational modifications, as exemplified by the biarylitides. These are a fam