N-乙酰鸟氨酸脱乙酰基酶(N-Acetylornithine deacetylase, NAO)是一种重要的用于手性拆分的酶,具有广泛的底物选择性,常用于多种活性氨基酸的酶法拆分.采用稀释复性法研究了重组NAO包涵体的复性条件,如蛋白浓度,复性液中尿素浓度,pH,GSH浓度及c(GSH)/c(GSSG)比例,同时对稀释操作方式进行了考察,得到了较为适宜的复性...
n-acetylornithine-deacetylase 例句 释义: 全部 更多例句筛选 1. Effects of Metal Ions on the Activity of Soluble Recombinant N-acetylornithine Deacetylase 金属离子对重组N-乙酰鸟氨酸脱乙酰基酶可溶部分酶活力的影响 www.ilib.cn© 2024 Microsoft 隐私声明和 Cookie 法律声明 广告 帮助 反馈...
关键词:N-乙酰鸟氨酸脱酰基酶,活性中心,化学修饰,底物保护 中图分类号:Q556 文献标识码:A 大肠杆菌中 argE 编码的 N-乙酰鸟氨酸脱酰基酶(N-acetylornithine deacetylase,简称 NAOase;EC3.5.1.16)催化 N-乙酰鸟氨酸脱去乙酰基,生成 L-鸟氨酸。该反应是精氨酸合 成关键的一步[1-2]。NAOase 可应用于氨基酸...
7E) in the acetylornithine deacetylase structure from Rhodopseudomonas palustris suggested that the negatively charged ASP-110 as well as MET-111 are directly involved in the binding. The structural model of mNAOD devoid of the zinc binding residues, when superimposed over the full length NAOD has...
ArgE 编码的N-乙酰鸟氨酸脱酰基酶(N-acetylornithine deacetylase, NAOase; EC3.5.1.16) 催化N-乙酰鸟氨酸脱去乙酰基,生成L-鸟氨酸。该反应是精氨酸合成途径的关键,产物鸟氨酸是多胺类物 质合成的前体 [2-4] 。多胺和DNA 复制及细胞分裂有关,故NAOase 的活性对细菌繁殖不可缺少,argE 基因 及其编码的NAOase...
描述N-Acetylornithine 是从 L-谷氨酸到 L-精氨酸的酶促生物合成中的中间体。 相关类别 天然产物>>其他 研究领域>>其他 靶点 Human Endogenous Metabolite N-alpha-乙酰基-L-鸟氨酸物理化学性质 密度1.171g/cm3 沸点436.2ºC at 760 mmHg 分子式C7H14N2O3 ...
[5]. Coenzyme A andN-acetylglutamate also inhibit the activity of this enzyme: 2.5 mM CoA or 25 mM N-acetylglutamate both result in 50% inhibition of ArgA [6]. InE. coli, removal of the acetyl group is catalysed by ArgE (N-acetylornithine deacetylase) during the fifth step of ...
[PDF] Involvement of the Putative N-Acetylornithine Deacetylase from Arabidopsis thaliana in Flowering and Fruit.pdf 2015-06-09上传 暂无简介 文档格式: .pdf 文档大小: 2.03M 文档页数: 13页 顶/踩数: 0/0 收藏人数: 0 评论次数: 0 文档热度: ...
We have identified in Xanthomonas campestris a novel N -acetylornithine transcarbamylase that replaces ornithine transcarbamylase in the canonic arginine biosynthetic pathway of several Eubacteria. The crystal structures of the protein in the presence and absence of the reaction product, N -acetylcitrul...
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