Thus, I pick up some major actin-binding proteins expressing in nerve cells, and summarize (i) how these proteins affect actin properties, and (ii) possible functions of these proteins in nervous system. Finally, both conventional and unconventional myosins expressing in nerve cells are also ...
Fesselin is an actin binding protein that bundles actin filaments and accelerates nucleation of actin polymerization. The effect of fesselin on actin polymerization is regulated by Ca ++ -calmodulin. Because actin filaments serve both structural and contractile functions we also examined the effect of...
The actin-binding property of the myosin head 20 K (K = 103 Mr) fragment has been examined by a structural assay. A new fragment is produced by digestion of scallop myosin synthetic filaments with a lysine-specific protease. This fragment consists of the rod together with two "nubs" corresp...
In order to understand the molecular mechanism of muscle movement or cell motility, identification and characterization of actin-binding sites on myosin head and myosin-binding sites on actin molecules are necessary. Elucidation of the correlation between the binding sites and the myosin ATPase reaction...
Anillin binding and diffusion assay Actin filaments stabilised by biotin-phalloidin were attached to anti-biotin antibodies on a glass coverslip. The channel was then washed with twice the channel volume of HEPES-based imaging buffer (20 mM HEPES, 2 mM MgCl2, 1 mM EGTA, pH 7.2 KOH, ...
Myosin motor proteins convert chemical energy into force and movement through their interactions with nucleotide and filamentous actin (F-actin). The evolutionarily conserved lysine-265 (K265) of the myosin-2 motor from Dictyostelium discoideum (Dd) is p
Muscle - Actin-Myosin, Regulation, Contraction: Mixtures of myosin and actin in test tubes are used to study the relationship between the ATP breakdown reaction and the interaction of myosin and actin. The ATPase reaction can be followed by measuring the
Cardiac myosin binding protein-C (cMyBP-C) is a thick filament protein that interacts with titin, myosin and actin to regulate sarcomeric structure and regulation of acto-myosin cross-bridge cycling (Fig. 1) [51,52]. Mutations in cMyBP-C gene MYBPC3 are the most abundant HCM gene...
The rigor complex of actin and trypsin-treated myosin subfragment 1 (S1) whose heavy chain was cleaved into three fragments (20K, 25K, and 50K) was cross-linked with a zero-length cross-linker, 1-ethyl-3-[3-(dimethyl-amino) propyl]carbodiimide. The cross-linking reaction generated three ...
The conformational elasticity of the actin cytoskeleton is essential for its versatile biological functions. Increasing evidence supports that the interplay between the structural and functional properties of actin filaments is finely regulated by actin-binding proteins; however, the underlying mechanisms and...