We purchased this antibody for immunostainning and western blot. It works very well for both the applications in cultured myoblasts. 使用Google 翻译 最初发布在 MyoD 抗体 (G-1) 是否有用? 是· 0 否· 0 报告 ★★★ ★★★ 4星,共 5 星。 hg2017 · 7年前 good ab I got a fre...
Western Blot (WB) 免疫原 免疫原详情,请参考产品官网页面信息 来源宿主 Rabbit 反应性 人, 小鼠, 大鼠 保存建议 This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C...
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen Form Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Gene Name MYOD1 Accession No. P15172 P10085 Q02346 Alternate Names MYOD1; BHLHC1; MYF3...
Efficiency of lentivirus infection was confirmed with Western blot using MYOD antibody (Ab) as well as eGFP transduction rate. The transduction rate was approximately 80% of infected hAFS cells. BALB/cSlc-nu mice were anesthetized with intramuscular injection of 80 μl of a solution (33.8% ...
(1 × PBS and 5 mg/ml BSA-fraction V) at R.T. for 3 h or at 4 °C overnight. The beads were then added to the chromatin/antibody and incubated for 4 h at 4 °C with rotation. Next, the beads were washed twice with cold low-salt wash buffer (20 mM Tris pH ...
(e) Vinculin expression level calculated by western blot technique: actin production is reduced in cells grown into the Nichoid. (f) Number of focal adhesions per cell. Spread cells present a high number of traction forces sites respect to the cells grown into the Nichoid. (g) Focal ...
Transcripts per Million.nWestern blot of RD, JR1, and SMS-CTR cells treated with 10 nM trametinib compared to vehicle control (DMSO), probed for p-ERK (phosphorylated), ERK, SNAI2, MEF2C, and actin (loading control). Representative blot,n = 3 biologically independent experiments.oSc...
(DSHB, Iowa City, IA, USA) at 4 °C overnight. After washed with PBS, the cells were treated with FITC-conjugated anti-rabbit IgG antibody and incubated in dark for 1 h. The cell nuclei were stained with DAPI (Beyotime, Jiangsu, China). The total myotube area was calculated and...
After washed with PBS, the cells were treated with FITC-conjugated anti-rabbit IgG antibody and incubated in dark for 1 h. The cell nuclei were stained with DAPI (Beyotime, Jiangsu, China). The total myotube area was calculated and measured as previously described.50 The 5′- and 3′-...
Coimmunoprecipitated HDAC1 (both endogenous and exogenously transfected) was revealed by Western blot using anti-HDAC1 antibody. One-tenth of the total lysate before the immunoprecipitation was used to monitor the expression levels of MyoD and HDAC1 and the phosphorylation status of pRb. (C) ...