Cited in 107 publications. Myelin basic protein antibody for WB, IHC, IF-P, ELISA and reacts with human, mouse, rat.
Myelin has a unique composition, with 70–80% being lipids, including cholesterol, plasmalogens and galactosylceramide (GalCer), and a small set of proteins such as myelin basic protein (MBP) and proteolipid proteins (PLP)1. The intricate process of synthesizing and transporting large amounts of...
where lipid transfer proteins solubilize lipids, shielding them from the aqueous phase as they move between membranes. GLTP is a cytosolic protein known to interact with vesicle-associated membrane proteins (VAP-
After being blocked in 10% normal blocking serum (Sigma, USA) at room temperature for 15 min, the slides were incubated with rabbit antibody against E-cadherin (1:150) (Cell Signalling Technology; USA) and rabbit antibody against Vimentin (1:100) (Proteintech, USA) at 4 °C over...
(60004, 1: 10,000, Proteintech). After incubation with secondary HRP-labeled antibodies (Proteintech), protein bands were detected by the ECL chemiluminescence detection system. Imaging of bands was performed with an Image-Quant LAS4000 mini image analyzer (GE Healthcare). The bands of Western ...
(Proteintech); mouse anti-GM130 (610823, dilution 1:1000) (BD Biosciences); sheep anti-TGN38 (AHR499G, dilution 1:1000) (Bio-Rad); Goat Anti-Rabbit IgG(H + L) Mouse/Human ads-HRP (4050-05, dilution 1:10,000) (Southern Biotech); Alexa 488-, 594-, or 647-conjugated ...