The Coombs test, or direct antiglobulin test, detects excessive antibody or complement bound to red blood cells' surfaces and is the standard assay for immune-mediated hemolytic anemia. Flow cytometry and immunofluorescent antibody tests may also be used to detect autoantibody bound to erythrocytes ...
Peripheral blood (2 mL) was collected from each participant from elbow venous. Serum was isolated from peripheral blood with saturated (NH4)2SO4 as previously described (Zeng et al. 2012). The isolated sera were concentrated with ultrafiltration centrifuge tubes (Millipore, Billerica, MA, USA) ...
pneumoniae has been recovered from or identified by PCR in the blood, pericardial fluid, middle ear fluid, vesicular skin lesions, pleural fluid, kidneys, brain, and cerebrospinal fluid (CSF).* In addition, the observation of low CSF glucose in M. pneumoniae meningoencephalitis suggests direct ...
Positivity in Serodia‐Myco II and LISA IgG was associated with blood group or Coombs positivity, suggesting non‐specific reactions. The study confirmed that single serum serology is unsuitable for the diagnosis of M.?pneumoniae infection, and that commercially available tests need further improvement...
Of the 175 blood samples from adult birds (Gallus gallus domesticus), 74 (42.29%) - [34.87 to 49.97%] had positive serology for M. gallisepticum. Positive results were significant and show the need for preventive measures in small farms in the region studied. Key words: Poultry, mycoplasmo...
Blood samples were obtained at the time of enrolment and two to four weeks after. White blood cell (WBC) count, C-reactive protein (CRP) and serological tests were performed at enrolment. The patients were hospitalized on the basis of the clinical decision of the pediatricians on duty. Data...
Diagnostic test Nucleic acid amplification: PCR is the gold standard for diagnosing M. pneumoniae infection due to its higher specificity than serology[8-12]. It is a quick and accurate method for diagnosing mycoplasma infections in children, commonly using oropharyngeal or nasopharyngeal samples. Its...
such as myocarditis, pericarditis, heart failure, and blood Systemic damage to the fluid system, skin, mucosa and motor system [12]. In recent years, Mpn can also be found in the reproductive system Systemic infection. STD related Mycoplasma species are more, in addition to Uu, ...
Patients and household contacts were asked to provide both throat samples and a blood sample for serology, whereas only throat samples were obtained from school children. Household contacts and school children answered questionnaires concerning disease symptoms at the time of sampling and during the prec...
This study aimed to compare a real-time PCR (rPCR) test with improved detection limit to serology and culture for the detection of Mycoplasma gallisepticum (MG) infection in chicken breeder flocks. Six hundred and forty-six blood and tracheal swab samples belonging to 31 grandparent chicken ...