These results are in agreement with Wyeth (34), who used the same MS strain through spray route in 42 days-old chickens. When the footpad route was used, Wyeth observed joint lesions, strong serologic response, and weak spreading of the used MS strain. Considering that all MS in...
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840 bp (pMmc-95010, GenBank accession number FQ790215). The chromosome has a G+C content of only 23.8 mole%. Genome annotation revealed 924 putative CDS, two Integrative Conjugative Elements (ICE), each being on average 30 kbp long and...
Validation of massive quantification by SWATH was performed with MRM methodology on QTRAP 4500 (Sciex, USA) triple quadrupole mass spectrometer equipped with a NanoSpray III ion source (Sciex) coupled to an expert NanoLC 400 nano-HPLC system (Eksigent, USA). Validation was performed with 3 indep...
The eluting peptides flowed into a MicroIonSpray II-mounted 75 μm ID emitter tip that tapered to 15 μm, ionized by nanoelectrospray with 2,300 V into the source of the QSTAR, which then performed an Information Dependent Acquisition (IDA) experiment. The MS/MS data files produced by ...
The delivery mode for the F strain vaccine is intranasal instillation or coarse spray and eye drops. In the early 1990 s, the 6/85 vaccine strain was developed in the USA. It is not virulent in chickens and turkeys, and no bird-to-bird transmissions have been reported. This vaccine ...