16549\nMYCOPLASMA-FREE HEPATOMA CELL LINE\nABSTRACT OF THE DISCLOSURE\nA hepatoma cell line which sheds HBsAg but\nwhich is free of mycoplasma is obtained by injecting\nimmuno-suppresed mice with cells from the hepatoma\ncell line and isolating cells from tumors which\ndevelop in the mice....
Mycoplasmas(commonly used trivial name for microorganism of the classMollicutes) are known to be one of the smallest free-living forms of bacteria that are broadly distributed in nature[1]. Based on their genetic background and molecular features,mycoplasmaswere hypothesized to originate evolutionarily...
Tenericutes / Mollicutes / Mycoplasmatales / Mycoplasmataceae / Mycoplasma Pleomorphic cells, 300–800 nm in diameter, varying in shape from spherical, ovoid or flask-shaped, or twisted rods, to slender branched filaments ranging in length from 50 to 500 nm. Cells lack a cell wall and are ...
The contaminated cultures differed from mycoplasma-free cells in cell distribution for chromosome number. The frequency of cells with modal chromosome number 21 decreased, while that of cells with 20 chromosomes increased. Decontamination of ... GG Polianskaia,LS Sizova,TN Efremova,... - 《Tsitolo...
1973. A whole cell mounting technique for detecting Acholeplasma laidlawii A on the surface of cultured cells. In Vitro (monogr.) Abstract 51, p. 413. Google Scholar Draghici, D. 1969. Comparison of primary cell cultures and cell-free media for the isolation of mycoplasma from pigs. ...
2. I have collected some cells with the supernatant that is to be tested, will this interfere with the assay? There is no need to worry, the presence of mammalian cell DNA will not affect the detection assay. 3. During sample preparation, is there an advantage to heating the sample to ...
Mycoplasmas are prokaryotes within the class Mollicutes and are the smallest free-living, self-replicating microorganisms.1,2 All members of the class lack a protective cell wall; thus they are damaged easily when outside of the host and are difficult to identify with most staining techniques. ...
Mycoplasmas Are Responsible for Interferon Induction in Cocultures of Mouse NK-Target Lymphoma Cells with Mouse Splenic Lymphocytes M Koi,MST Ebina,N Ishida - 《Microbiology & Immunology》 被引量: 5发表: 1983年 Studies on the mechanism of natural killer cell-mediated cytotoxicity. IV. Interferon-...
METHODS: Selected populations of viable, motile and infection-free human spermatozoa from three healthy men were incubated with 15-480 multiplicity of infection (MOI) units of DiIC18-labelled Mycoplasma hominis. Cells were analyzed by means of confocal microscopy and by the eosin-Y dye exclusion ...
Three main states are labelled: “In”, helix inserted in the membrane; “Out”, helix free in the solvent, detached from the membrane; and “Lay”, helix lying on top of the membrane fully exposed to the solvent. (C) Representative structures of the three main states. Full size image ...