tuberculosis H37Rv does indeed possess a functional cellulase. The x-ray crystal structure of this enzyme, in ligand complex forms, from 1.9 to 1.1A resolution, reveals a highly conserved substrate-binding cleft
Structure-based in silico approaches for drug discovery against Mycobacterium tuberculosis Abstract Mycobacterium tuberculosisis the causative agent of TB and was estimated to cause 1.4 million death in 2019, alongside 10 million new infections. Drug resistance is a growing issue, with multi-drug res...
Cell Host Microbe 29, 165–178.e8 (2021). Article CAS PubMed Google Scholar Plumlee, C. R. et al. Ultra-low dose aerosol infection of mice with Mycobacterium tuberculosis more closely models human tuberculosis. Cell Host Microbe 29, 68–82.e5 (2021). Article CAS PubMed Google Scholar ...
Mycobacterium tuberculosis (Mtb) forms biofilms harbouring antibiotic-tolerant bacilli in vitro, but the factors that induce biofilm formation and the nature of the extracellular material that holds the cells together are poorly understood. Here we show that intracellular thiol reductive stress (TRS) in...
Bacterial D is ulfide b ond forming (Dsb) proteins facilitate proper folding and disulfide bond formation of periplasmic and secreted proteins. Previously, we have shown that Mycobacterium tuberculosis Mt-DsbE and Mt-DsbF aid in vitro oxidative folding o
tuberculosis field. To build a whole picture, we will thus first present the structure of internal CRISPR-Cas and its possible roles on MTBC physiology. We will then discuss the technological developments based on CRISPR-Cas, for diagnostics of tuberculosis and for targeted genetic modifications in...
tuberculosis has been indirectly proposed based on in vitro results. There is no evidence however for TlyA relevance in the survival of tubercle bacilli inside host cells or whether both activities are functionally linked. A thorough analysis of structure prediction for this mycobacterial protein in ...
tuberculosis antigens, specifically ESAT-6, interfere with TCR/CD28-induced upstream as well as downstream signalling events which might be responsible for defective IL-2 production which further contributed in T-cell unresponsiveness, implicated in the progression of disease.To the best of our ...
infection, and highlight the strategies employed byM. tuberculosisto counter macrophage attack. Interestingly, research on the evasion tactics employed byM. tuberculosiswithin macrophages not only helps to design strategies to curb tuberculosis, but also allows a better understanding of host cell biology....
Presents research which described the structure of the MscL channel homolog from Mycobacterium tuberculosis by x-ray crystallography. Role of MscL large-conductance mechanosensitive channels in cell transduction; Organization of the channel; Effects of a water-filled opening from the extracellular side of...