Myc标签 tagged 释义 [医]标记的,加标记的,加了标记的 网络 标签; 标记的,示踪的; 示踪的 Customizations do not affect notes you've already tagged.自定义不影响已标记的笔记。
Binding capacity≥ 0.6mg Myc‐tagged fusion protein per ml beads SpecificityMet-Myc-Protein, Myc-Protein, Protein-Myc Elution methodAcid, peptide competitive or SDS‐PAGE loading buffer elution. Note: If elute with SDS-PAGE loading buffer, the light (~25kDa) and heavy (~50kDa) chain of Myc...
使用这些Myc-Tagged & HA-标签载体可以将目的蛋白质与c-Myc或者血凝素(HA)表位标签进行融合表达。这两种标签都具备良好表征和高度的免疫反应性,是免疫共沉淀研究的理想选择,同时也很容易通过Western Blotting的方法或者荧光显微镜进行检测(使用anti-myc 抗体Code No.631206)。此外,c-Myc和HA标签分子量小,通常不会干...
(1) GST标签:是谷胱甘肽S-转移酶,具体蛋白表达产率高,表达产物易于纯化、可溶等特性,常用于pull-down。(2) HA标签:源于人类流感血凝素的99-106位氨基酸序列,HA标签具有较强的免疫原性,在蛋白分离纯化,检测示踪中应用较多,HA标签抗体能特异识别C末端或N末端带有HA标签(HA-tagged)的融合蛋白.(3) GFP:绿...
Confocal immunofluorescent analysis of COS cells expressing a Myc-tagged protein (left) and untreated (right), using Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 647 Conjugate) (blue pseudocolor). Actin filaments have been labeled with DY-554 phalloidin (red). Show more Confocal im...
This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated Sepharose®beads. Myc-Tag (9B111) Mouse mAb (Sepharose®Bead Conjugate) is useful for the immunoprecipitation of Myc-tagged recombin...
The Thermo Scientific Pierce c-Myc Tag IP/Co-IP Kit provides the affinity resin and other reagents necessary to easily perform immunoprecipitation (IP) or co-immunoprecipitation (co-IP) experiments using a c-Myc-tagged protein as the bait.T
pEF1α-HA is a mammalian expression vector that constitutively expresses a protein of interest fused to an N-terminal hemagglutinin (HA) epitope tag, even after stable integration of the vector into the host cell genome. Stable, constitutive expression of the tagged protein is driven b...
Reagent compatibilityChaotropic reagents will denature the target Myc-tagged protein. Do not exceed 0.3M GuHCl or 1.5M Urea. ApplicationSuitable for IP、Co-IP and protein purification. Storage-20℃ 本试剂盒提供三种洗脱方法。根据目的蛋白的结构、生物学功能及后续应用的要求等,本试剂盒提供三种洗脱方法,...
Enhancer Box sequences (E-boxes) and recruiting histone acetyltransferases (HATs). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. This Myc-Tag antibody detects Myc-tagged fusion ...