Max: a helix-loop-helix zipper protein that forms a sequence-specific DNA-binding complex with Myc. Science 251, 1211–1217 (1991). Article CAS PubMed Google Scholar Amati, B. et al. Oncogenic activity of the c
The neuronal-specific RNA-binding Hu proteins are present in cytoplasmic and nuclear fractions of N cells and one of them, HuD, binds specifically to both exonic and intronic N-myc RNA sequences. In sense and antisense HuD-transfected N cells, there are coordinate changes in HuD and N-myc ...
mRNA stabilityc-myccoding region determinantRNA-binding proteincell-free extractsregulation endoribonucleasec-myc mRNA contains at least two discrete sequence elements that account for its short half-life, one in the 3′-untranslated region, the other in the carboxy terminal coding region (coding ...
C-Myc过表达在MM的发生和发展中起着关键的作用,目前认为MM中C-Myc过表达的机制有以5种:(1)染色体易位。涉及C-Myc异常表达的染色体易位情况在新诊断的MM患者中占20%～50%,这些易位涉及免疫球蛋白(immunoglobulin,Ig)基因位点和一些非Ig融合伴侣基因,如46序...
Additionally, these activities are 3′-UTR-dependent because the c-myc 3′-UTR directs 3′ to 5′ decay whereas β-globin mRNA sequences do not. We and others have hypothesized that in cells the poly(A) tract of c-myc mRNA is protected by PAB and that early events in the decay of...
Related antisense oligomers with 2- to 12-base-pair mismatches with c-myc mRNA did not influence HL-60 cells. Thus, the effects of the antisense oligomer exhibited sequence specificity, and furthermore, these effects could be reversed by hybridization competition with another complementary oligomer...
HL-60 cells, a human promyelocytic leukemia cell line, contain amplified c-myc DNA sequences and mRNA transcripts. These cells can be induced to undergo macrophage differentiation by phorbol esters, which results in suppression of c-myc expression and cessation of cell proliferation. The nuclear ...
Thus, herein, we explored the binding effects of Curcumin and its various analogs with a biologically relevant G-quadruplex DNA sequence of the human c-myc promoter region, forming a G-quadruplex structure. Their interactions were studied using various spectroscopic techniques such as steady-state an...
leukemiawithanoligonucleotideencodingthegenomicPU27sequenceinducescellcyclearrest anddeathbyoncotic-necrosisduetoPU27-mediatedsuppressionofc-mycmRNA/protein expression.Furthermore,PU27isabundantlytaken into cells, localized in the cytoplasm/nucleus, inherently stable in serum and intracellularly, and has no effect...
最后,通过qRT PCR分析40例高危HPV16\/18\/31\/33\/45的E6\/E7 mRNA阳性宫颈拭子和40例高危HPV阴性样本中miR-34a\/b\/c和miR-146a-5p的表达。在E6\/E7阳性样本中,miR-34a和miR-146a-5p的水平显著低于E6\/E7阴性样本,...