PsbA, PsbS, PsaC, and LHCA proteins were detected with antiLHCA1 antibody (1:4000 dilution) (# AS01 005, Agrisera), PsbA (1:4000 dilution) (# AS05 084, Agrisera), PsbS (1:4000 dilution) (# AS09 533, Agrisera), PsaC (1:4000 dilution) (# AS10 939, Agrisera) and Goat anti-...
Corrected isogenic control of IVS27+1G>A was generated by base editing. To evaluate UPS activity, we employed ubiquitin fusion degradation reporter, Ub-G76V-GFP, which provides fluorescence readout of UPS activity. BNP expression of hiPSC-CMs were evaluated by antibody labeling and fluorescence-...
The cells were then incubated with a primary antibody for 1 h, followed by incubation with the appropriate secondary antibody for 1 h at room temperature. The samples were analysed using an Eclipse 80i fluorescence microscope (Nikon) or an LSM 700 confocal microscope (Carl Zeiss). Images ...