通过对其定义和特点的阐述,读者将更加清晰地了解这一元件的功能和作用机制,从而对其在生物学领域中的重要性和价值有更深刻的认识。 2.探索myb-like sequence顺式作用元件的功能和作用机制。通过对其功能和作用机制的详细解析,读者将深入了解该元件在基因调控中的作用过程,从而为相关领域的研究提供理论和实践的依据。
另外,植物 MYB 还能够与构成 ABA 信号转导网络 的成员间形成调控通路,在盐胁迫下调节 ABA 信号 稳态.例如,在苹果叶片表皮保卫细胞中高表达的 MdMYB44-like, 能够直接结合 MdPP2CA 启动子区的 MBS 元件,抑制其转录过程 ;MdMYB44-like 与 ABA 受体蛋白 MdPYL8 互作会显著增强这种转录抑 制作用,植株迅速积累 ...
In addition,the phylogenetic tree showed that its amino acid sequence shared 62.7% identity with Ricinus communis that belonged to the same family of Euphorbiaceae. qRT-PCR expression analysis revealed that JcMYB308 expressed in different organs,abundantly in root,but scarcely in leaves,and at the ...
本发明公开了一种苹果MYB转录因子MdMYB90‑like及其应用。所述苹果MYB转录因子MdMYB90‑like的氨基酸序列如SEQIDNO:1所示。所述苹果MYB转录因子MdMYB90‑like作为转基因标记在筛选转基因材料中的应用。所述苹果MYB转录因子MdMYB90‑like在调控花青素合成中的应用。
因此,本研究后续可分析 PcMYB1 与下游基因启动 子区的 AC 元件的互作,进一步研究 PcMYB1 调控 木质素合成的分子机制. 本研究证明转录因子 PcMYB1 具有转录抑制 活性,PcMYB1 在虎杖根,茎,叶中均有表达.与 野生型拟南芥相比,转基因拟南芥(35::PcMYB1) 株系出现植株矮化现象,木质部细胞层数变少且木 质素含量...
Sequence-specific DNA recognition by the Myb-like domain of plant telomeric protein RTBP1. J Biol Chem,2000, 275: 24208−24214. [13] Dai X, Xu Y, Ma Q, Xu W, Wang T, Xue Y, Chong K. Overexpression of an R1R2R3 MYB gene, OsMYB3R-2, increases tolerance to freezing, drought,...
pigment in fruits is derived from anthocyanin.With the completion of genome sequence of most fruits,the important advances have been acquired in the molecular mechanism of fruit coloring.In particular,the mechanism of transposons acting as important regulatory element in control of fruit coloration has ...
顺式元件 Cis-element典型序列 Typical sequence特性 Characteristic基因 Gene ABREACGTG脱落酸响应 Abscisic acid responsivenessSiNADP-ME1,2,4,5,6,7 CACGTGSiNADP-ME2,4,6 GCCGCGTGGCSiNADP-ME1,4,6 TCA-elementTCAGAAGAGG水杨酸响应 Salicylic acid responsivenessSiNADP-ME4,7 ...
metabolism and responses to biotic and abiotic stresses . R2R3-MYB transcription factors can be divid- ed into 25 subfamilies based on the conserved amino acid sequences. In these subfamilies, the 22nd subfamily that responses to biotic and abiotic stresses includ AtMYB44 , AtMYB77 , AtMYB7...
以v型紫斑白三叶的cdna为模板,使用设计的特异扩增引物trmyb308-f和trmyb308-r进行pcr扩增,得到包含部分5'-utr和部分3'-utr在内的trmyb308全长cds(codingsequence)序列(结果未显示),与rna-seq筛选所获得的片段大小(897bp)基本一致。生物信息学分析发现,该基因的orf(openreadingframe)长度为921bp(seqidno.2),编...