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Lysates (30 μg/lane) were loaded and run on 10% Tris-Glycine gels (Biorad), transferred to nitrocellulose membrane and Western blot analyses conducted as described in (Aizen et al., 2016). The membranes were probed with a GAL4 Antibody (DBD) (#sc-577 Santa Cruz) and visualised on ...
The two constructs were introduced into onion epidermal cells with a Biolistic Particle Delivery System (BioRad). The fluorescence of GFP in onion epidermal cells was visualized under a Confocal mi- croscope (Olympus FV500). Transcriptional activation activity of the AtMYB59 protein The coding ...
Lignocellulosic biomass represents a renewable and sustainable resource for the production of biofuels and bio-based chemicals. The most common approach for lignocellulosic biomass utilization is biochemical conver- sion, where enzymes are utilized for breaking down structural polymers in plant biomass to ...
Between 20-40μg of samples were run on NuPAGE Bis-Tris 4%–12% gels in NuPage MES SDS Buffer (Invitrogen) for 40 minutes at 200 V and transferred to 0.4-μm nitrocellulose membranes using a semi-dry transfer system (Bio-Rad Catalog #1704150) at 1.3 A and 25 V for 15 minutes. ...
(Bio2Rad),电泳槽(Bio2Radmini2subcellGT);紫外 pigsmaybeconnectedwithcontrollingofintron.Thevariationof155nucleotideallweredetectedincodingandnoncodingre2 gionbycomparingJuemapigwithother14breeds,and119outofthemwereinintronregionsand28outofthemwereinexon ...
Western blotting was performed as previously described30. Total protein from cells (10 μg) and exosomes (10 μg) was fractionated using an electrophoretic gradient across Mini-PROTEAN tris-glycine extended gels (BIO-RAD, Richmond, CA, USA). Measurement of protein concentration was performed ...
After washing, the blot was exposed to a PhosphorImager screen (Fuji) for 3 h to overnight, and the screen was scanned using a Molecular Imager FX (Bio-Rad, Hercules, CA). The image was processed using the ImageQuant software (GE Healthcare, Piscataway, NJ). RNA ligase-mediated 50 RACE...