Cancer stem cellsVery small embryonic-like stem cells (VSELs)Multiple theories exist to explain cancer initiation, although a consensus on this is crucial for developing effective therapies. 'Somatic mutation theory' suggests that mutations in somatic cells during DNA repair initiates cancer but this ...
not in every cell in the body. These changes can be caused by environmental factors such as ultraviolet radiation from the sun, or can occur if a mistake is made as DNA copies itself during cell division. Acquired mutations in somatic cells (cells other than sperm...
Nevertheless, somatic mutations in HEKa cells were detected using an orthogonal approach to the one applied for MEFs and HFFs by avoiding barrier-bypass-clonal expansion. Specifically, irradiated and control HEKa cells were grown for exactly 14 days (Fig. 1b), corresponding to approximately 8 ...
et al. Somatic mutations of calreticulin in myeloproliferative neoplasms. N. Engl. J. Med. 369, 2379–2390 (2013). CAS PubMed Google Scholar Giustacchini, A. et al. Single-cell transcriptomics uncovers distinct molecular signatures of stem cells in chronic myeloid leukemia. Nat. Med. 23, ...
Broadly, mutations fall into two categories — somatic mutations and germline mutations — according to the authors of “An Introduction to Genetic Analysis, 7th Ed” (W.H Freeman, 2000). Somatic mutations occur in their namesake somatic cells, which refers to the various cells of one’s body...
The somatic mutation theory of aging posits that the accumulation of mutations in the genetic material of somatic cells as a function of time results in a decrease in cellular function. In particular, the accumulation of random mutations may inactivate genes that are important for the functioning ...
Somatic mutations, either spontaneous or produced by identifiable mutagens, are thought to be important in the aetiology of cancer and in the ageing process. The study of somatic mutations in human cells in vivo has recently been made possible by the development of techniques for enumeration and ...
As a normal control, the genomic DNA of white blood cells in sediments was extracted using the DNeasy Blood & Tissue Kit (Qiagen Cat. No. 69504). The DNA concentration was quantified using the dsDNA HS assay kit on a Qubit 3.0 fluorometer (Life Technology, US) according to the ...
The accumulation of somatic DNA mutations over time is a hallmark of aging in many dividing and nondividing cells but has not been studied in postmitotic human cardiomyocytes. Using single-cell whole-genome sequencing, we identified and characterized the landscape of somatic single-nucleotide variants...
Tumours arise and develop via somatic mutations that confer a fitness advantage on cells1. Such driver mutations exert their phenotypic effect by altering the function of genes or genomic elements, and are characterised by signatures of positive evolutionary selection2. Tumour genomes also carry numerou...