Communications Biology volume 3, Article number: 472 (2020) Cite this article 3983 Accesses 20 Citations 1 Altmetric Metrics details Abstract In depth studies of quantitative trait loci (QTL) can provide insights to the genetic architectures of complex traits. A major effect QTL at the distal ...
S1b). Similar to the RE model, a P value is obtained by comparing the number of simulations with an exonic mean functional score higher or equal to the observed value (Formula 2). This module work with any base-level scoring method. Given its previous successful use and integrative nature...
we must show that heteroplasmic mtDNA mutations(1)can be reliably detected in single cells,(2)are propagated in daughter cells,(3)can be used to accurately determine clonal relationships,(4)can be combined with cell state measurements to learn meaningfulbiology, and(5)can be applied to study ...
- 《Genome Biology》 被引量: 21发表: 2018年 Efficient CRISPR/Cas9-mediated multiplex genome editing in CHO cells via high-level sgRNA-Cas9 complex Increasing demand for recombinant therapeutic proteins has warranted the need for an efficient host cell to produce high-quality proteins, with a high...
Population-level dynamics of mutations in 10 frequently hit genes show consistent patterns. For each panel,achemostat 1,bchemostat 2, andcchemostat 3, the elapsed number of generations is depicted on thex-axis; the height of each gray box within each panel represents a frequency of 100%. Cum...
- 《Journal of Molecular Biology》 被引量: 63发表: 1991年 downloaded from journal of bacteriology, role of tnra in nitrogen source-dependent repression of bacillus subtilis glutamate synthase gene expression The DNA binding protein, GlnR, encoded by glnR, is believed to be directly responsible ...
4 Coding mutations influence the mRNA level of the mutated gene. a, Non-significant negative correlation between the mean fitness of synonymous mutants of a gene and the expression level of the gene. Each dot represents a gene. Spearman’s correlation ρ and associated P-value are presented. ...
a... C Délye,F Pernin,L Scarabel - 《Plant Science An International Journal of Experimental Plant Biology》 被引量: 89发表: 2011年 Genetic Analysis of Imidazolinone Resistance in Mutation-Derived Lines of Common Wheat The imidazolinone herbicides possess high biological potency at low ...
Recent studies combine two novel technologies, single-cell RNA-sequencing and CRISPR-Cas9 barcode editing for elucidating developmental lineages at the whole organism level. While these studies provided several insights, they face several computational challenges. First, lineages are reconstructed based on ...
A variant RNA containing an AUUAAA was polyadenylated at 20% the level of wild-type substrate RNA, while all other derivatives tested were not functional in vitro. The AUUAAA variant RNA formed specific complexes in native polyacrylamide gels and crosslinked to the AAUAAA-specific 64kd ...