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Muller in 1928 stressed that the gene has one unique property without which evolution could not have taken place and introduced the concept of mutation rate. Treatment with mutagens alters genes or breaks chromosomes. Gene mutations occur naturally as errors in DNA replication. Most of these errors...
The fungal strain Fol 1322 was obtained from the ICAR-Indian Agriculture Research Institute, New Delhi. The fungal culture was grown for 5 days at 25 °C in potato dextrose agar (PDA). For the pathogenic leaf disc assay, 10–15 large expanded leaves of 1-month-old tomato cv. AV were ...