d Mean fluorescence intensity (MFI) of inflammatory lesions in sections immunolabeled for LIF. * Indicates significant difference from WT/mdx (n = 4; P < 0.05). e–j Cross-sections of muscles from WT/mdx and LIF/mdx mice were immunolabeled with antibodies to F4/80 (e) and CD...
Details of the nervous system ofLobatocerebrum riegerin. sp. as seen with CLSM. DAPI in cyan, serotonin in red, FMRFamide in purple. The use of “glow” depends on the figure and is indicated for each of them. All images are maximum intensity projections of a subset of the original ima...
Mutations inASAH1have been linked to two allegedly distinct disorders: Farber disease (FD) and spinal muscular atrophy with progressive myoclonic epilepsy (SMA-PME). We have previously reported FD-like phenotypes in mice harboring a single amino acid substitution in acid ceramidase (ACDase), P361...
The percentage of Casp3+/SMI32 + co-labeled MNs quantified by immunohistochemical analysis was reduced in SMA mice treated with ASC-EVs (10.59 ± 2.36%) compared to the SMA-PBS group (21.48 ± 3.47%). WT animals showed a statistically lower expression of Casp3 (2.51 ...
The TA muscles from wild-type and mdx mice treated with rMsGal-1 or PBS were stained with Hematoxylin and Eosin or Alexafluor-488 labeled Wheat Germ Agglutinin and 4',6-diamidino-2-phenylindole (DAPI) (Figure 6a) and analyzed for the presence of CLN (Figure 6b) and Feret's diameter (...
Briefly, the RNA was amplified and labeled with Cy3 using the Agilent Low Input Quick Amp Labeling Kit (Agilent Technologies) according to the manufacturer’s protocol. The hybridization was performed according to the Agilent 60-mer oligo microarray processing protocol using the Agilent Gene ...
demyelination, loss of axons, and altered sphingolipid levels in P361R-SMA spinal cords; severe pathology was restricted to the white matter. Our model can serve as a tool to study the pathological effects of ACDase deficiency on the central nervous system and to evaluate potential therapies ...
Interestingly, tracingPax3+GFP-labeled MABs also revealedPax3+cells in postnatal blood vessels. Although their frequency appears to be lower than the embryonic correlate, thesePax3+progenitors, called pericytes, are considered the descendants of the multi-potentPax3+cells present during embryogenesis [...
Figure 2 Gene-based manhattan plot of genetic associations with grip strength in discovery sample. The most significant gene for each chromosome is labeled. Full size image To identify candidate genes regulated by the 64 replicated grip strength variants, we used the Genotype-Tissue Expression (GTEx...
(B) automatically labeled for artifact rejection through Multiple Artifact Rejection Algorithm (MARA), which is an algorithm trained using selected expert ratings74,75. Consequently, it can detect eye-, muscle-, and loose electrode artifacts. All components containing artifacts were excluded from the ...