we performed snRNA-seq and snATAC-seq on gastrocnemius (GAS) tissue from normal and denervated (14 days) male mice. In parallel, we performed bulk RNA-seq and H3K27ac ChIP-seq in mouse GAS muscles under matched conditions (Fig.1a). We constructed an atlas of snRNA-seq for both ...
Lastly, the activity of these agents was assessed in a muscular atrophy model, which was recreated by suspending the hindlimb of the mice for two weeks. Muscle tissue weight reduction was seen in the vehicle-treated muscular atrophy group compared to the non-suspension control group, which was ...
Under a Creative Commons license open accessHighlights Summary Regeneration requires both potential and instructions for tissue replacement. In planarians, pluripotent stem cells have the potential to produce all new tissue. The identities of the cells that provide regeneration instructions are unknown. He...
Collect- ively, the data generated by the in vivo muscle injury protocol showed that under an acute condition, SCs in this mouse model of Pompe disease are able to properly activate and efficiently contribute to muscle tissue repair. Loss of large fibers and splitting are typical features of ...
1A). Nano-sized HAp was observed on the surface of the vascular cells by scanning electron microscope (SEM) (Fig. 1B). Energy dispersive spectrometer (EDS) of the dense structures under SEM revealed calcium and phosphorus, with small amounts of magnesium and carbon (Fig S2). The ratio of...
To complete the data obtained from whole muscle tissue, glycogen accumulation was investigated at the scale of the single fiber using free-labeled TA muscle sections from 1.5- and 9-mo-old Gaa−/−and WT mice using the infrared IR microspectroscopy approach. As shown in Fig.2a, the bands...
Poly(lactic-co-glycolic acid) (PLGA);Microtube Array Membrane (MTAM);electrospinning;Tissue Engineered Vascular Grafts (TEVG);Smooth Muscle Cells (SMCs);Human Endothelial Vein Cells (HUVECs) 1. Introduction In this modern era, the sedentary life style has significantly contributed to a myriad of...
Top-hat filtering, followed by manual thresholding on the gray level, allowed segmentation of the connective tissue network (perimysium and endomysium). 4.7. Capillary Network Cross-sections were labeled with anti-CD31 (M0823 from Dako, Glostrup, Denmark), co-labeled with anti-laminin-α1, ...
Paraffin-embedded tissue sections were deparaffinized and hydrated on glass slides. Subsequently, the slides were immersed in a solution of Sirius Red dye for 1 h, followed by a 30-second rinsing with running water and dehydration using ethanol. After clearing the slides with a xylene-based cle...
The CMAP is a clinical assessment of neuromuscular function that is widely used to examine a range of motoneuron and muscle diseases because it indicates the sum of excitable tissue, although it does not necessarily indicate the numbers of motoneurons per se. Interestingly, both the vastus ...