Adult stem cells are indispensable for tissue regeneration, but their function declines with age. The niche environment in which the stem cells reside plays a critical role in their function. However, quantification of the niche effect on stem cell function is lacking. Using muscle stem cells (Mu...
electrical impulse through cardiac tissue, which is dependent on both the active membrane properties of the individual cardiac myocyte (i.e., electrical excitability and refractoriness) and passive properties governing the flow of current between cardiac cells (cell-to-cell coupling and tissue geometry...
4a), suggesting that the freeze–thaw method is not suitable for muscle tissue samples of sub-optimal quality. To investigate whether other tissue processing methods would improve the detection of CWD prion-seeding activity in RT-QuIC, given the sub-optimal tissue preservation described above, we ...
Rubinstein and Kelly (1980) tried to unravel the nature of the nervous influence in tissue culture experiments. Myotubes from embryonic hindlimbs of rats synthesize in vitro the fast type of myosin, but when stimulated at an appropriate frequency, they switch over to the production of slow ...
Skeletal muscle is the largest tissue in the body and plays a pivotal role in movement, posture, and energy metabolism [1,2]. During childhood, skeletal muscle undergoes rapid growth and development, which is critical for overall growth and development, as it provides the necessary strength for...
Western blot analysis of tissue homogenates showed no age- or alcohol-induced change in the amount of total eIF4E (Figure 3B). However, the function of eIF4E can also be controlled by its binding to a family of cap-dependent translational repressors, of which 4E-BP1 is the most prominent ...
Platelet-derived growth factor-BB (PDGF-BB) has been implicated in the proliferation, migration and synthetic activities of smooth muscle cells that characterize physiologic and pathologic tissue remodeling in hollow organs. However, neither the molecular basis of PDGFR-regulated signaling webs, nor the...
The protein disulfide isomerase family member ERp57 is one of the main up-regulated proteins in tissue of ALS patients and mutant SOD1 mice, whereas point mutations in ERp57 were described as possible risk factors to develop the disease. ERp57 catalyzes disulfide bond formation and isomerization ...
(NMJ accessory) with RNAscope probes, we observed groups of nuclei with co-localization of these transcripts in aged donor tissue sections, which were rare in young ones (Fig.3k). NMJ accessory also expressed more slow-twitch rather than fast-twitch MF markers (Extended Data Fig.5d), which...
Muscle biopsies were freeze-dried for 48 h and dissected free of visible blood, fat, and connective tissue. Muscle homogenates were generated as previously described27. Lysates were recovered by centrifuging the homogenates (20 min, 18,320g, 4 °C). Homogenate and lysate protein content ...