The diaphragm is a unique skeletal muscle due to its continuous activation pattern during the act of breathing. The ontogeny of macrophages, pivotal cells for skeletal muscle maintenance and regeneration, is primarily based on two distinct origins: postn
Briefly, total RNA (1 µg) was retro-transcribed using the Script Reverse Transcription Supermix (Bio-Rad, Hercules, CA, USA). RT-qPCR was performed using the g SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) and the CFX96 Touch Real-Time PCR Detection System (Bio-Rad). All ...
Mice C57BL/6 J male mice of 8 weeks old were housed at a controlled temperature of 24 ± 2 °C and relative humidity of 45 ± 15% with a 12 h light/dark cycle. Mice were randomized into four groups (n = 8) and intraperitoneally injected accordingly for 10 days:...
Axygen, USA) according to the manufacturer’s instructions. RNA from the serum was extracted using RNA extract reagent (AP-MN-BL-RNA-50, Axygen, USA). RNA was then reverse transcribed into cDNA using a Prime-Script RT reagent kit (RR047A, Takara, Japan). cDNA was amplified ...
Supplementary Figure Legends Table S1 Table S2 Figure S1 Figure S2 Figure S3 Figure S4 Rights and permissions Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or forma...
Abstract Experimental cell therapies for skeletal muscle conditions have shown little success, primarily because they use committed myogenic progenitors rather than true muscle stem cells, known as satellite cells. Here we present a method to generate in vitro-derived satellite cells (idSCs) from skele...
nuclease-free water and allowed to dissolve at 4 °C for 1 h before being stored at −20 °C. First, strand cDNA synthesis was performed using iScript RT Supermix from Bio-Rad (Saint-Laurent, QC, Canada) according to the manufacturer's instructions. All primers used are appended...