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Otherwise specifically indicated as “coventional FISH” in the figure legends, all the FISH experiments were single-molecule FISH using RNAscope. RNAscope_V2 kit was used according to the manufacturer’s instructions (ACD/bio-techne). We used Proteinase IV. When combined with antibody staining,...
Studies were conducted in a non-blinded fashion. For all studies, samples were not randomized, and sample size (range fromn=3 ton=14) are explicitly stated in the figure legends. Normal distribution was assessed by the Jarque–Bera test (P>0.05). All differences were analysed by analysis of...
Sample size or replicate number (designated as ‘‘n’’) for each experiment are indicated in the figure legends. Statistical analyses and graphing were performed using GraphPad Prism 6.0 (GraphPad Software). All experimental data are represented as mean ± standard deviation. Statistical significance...
Supplementary Figure and Table Legends (DOCX 17 kb) Rights and permissions Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this ar...