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Muscle cellsare typically large cells, some 20–100μm in diameter and many centimeters long, with the longest fibers being about 12cm. These cells aremultinucleated, because they need many nuclei to governprotein synthesisand degradation. The nuclei are typically located near the periphery of the...
Cancer growth induces hyperproliferation of Pax7-positive muscle stem cells, named satellite cells (SC), that do not fuse with myofiber and triggers an atrophy program whose insights are still unclear. When exposed to an increased TGFβ signaling, satellite cells express Twist that induces myostatin...
Immunofluorescence analysis of Smooth Muscle Actin was performed using 70% confluent log phase C2C12 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. ...
Muscle-invasive bladder cancer (MIBC) is a highly aggressive disease with a poor prognosis. B cells are crucial factors in tumor suppression, and tertiary lymphoid structures (TLSs) facilitate immune cell recruitment to the tumor microenvironment (TME).
protein expression variation in muscle cells in response to exercise and their receptors, genes that encode them , as well as the screening method using themPROBLEM TO BE SOLVED: To obtain an enzyme protein having an variable expression amount in association with motion strength, a secretory ...
Skeletal muscle stem cells (MuSC), also called satellite cells, are indispensable for maintenance and regeneration of adult skeletal muscles. Yet, a comprehensive picture of the regulatory events controlling the fate of MuSC is missing. Here, we determin
Schwann cells are well-known precursors of nerve regeneration [28]; with the advantage of a mouse model carrying the GFP under the control of S100 protein, a specific Schwann cell marker, infiltration of green cells was identified 90 days post-surgery from the surrounding native muscle to ...
Vascular smooth muscle cells (VSMCs) are highly phenotypically plastic, and loss of the contractile phenotype in VSMCs has been recognized at the early onset of the pathology of a variety of vascular diseases. However, the endogenous regulatory mechanism to maintain contractile phenotype in VSMCs...
Regeneration of skeletal muscle depends on a population of adult stem cells (satellite cells) that remain quiescent throughout life. Satellite cell regenerative functions decline with ageing. Here we report that geriatric satellite cells are incapable of