ZEB1Our previous study showed Musashi2 (MSI2) promoted chemotherapy resistance and pernicious biology of pancreatic cancer (PC) by down-regulating Numb and p53. We further explored the novel molecular mechanism involving its oncogenic role in PC development.We investigated the potential role and ...
we perform a genome-wide CRISPR/Cas9 screen. We identify TP53 and RNA-binding protein MUSASHI2 (MSI2) as the top-ranked sensitizer and driver of resistance to specific PRMT5i, GSK-591, respectively. TP53 deletion and TP53R248W mutation are biomarkers of resistance to GSK...
to date has explored the effects of MSI2 on ferroptosis, especially in CRC. Thus, the potential mechanism by which the RBP MSI2 regulates CRC ferroptosis deserves further investigation. Heat shock proteins (HSPs) are the most abundant molecular chaperone proteins; their aberrant expression is usuall...
We previously identified KrasLA1/+;P53R172HΔG/+ murine NSCLC cell lines expressing high (344SQ) versus low (393P) levels of MSI29. Using RPPA analysis of 171 total and phospho-proteins for expression changes, we examined protein expression changes associated with shRNA-mediated MSI2 knockdown...
We screened for stem cell identity-related genes preferentially expressed in a panel of cell lines with high versus low metastatic potential, derived from NSCLC tumors of Kras(LA1/+);P53(R172HΔG/+) (KP) mice. The Musashi-2 (MSI2) protein, a regulator of mRNA translation, was ...
(HER2) status and p53 mutations.19 In addition, downregulated DBC2 expression has been observed in lung, gastric, bone and bladder carcinomas.23–26 These findings indicate that DBC2 functions as both a tumor suppressor and a putative substrate- recruiting adaptor protein of the CUL3-based ...
目的探讨错配修复基因hMLH1,hMSH2及p53在子宫内膜癌组织中的表达及意义.方法运用免疫组织化学S-P法对99例子宫内膜癌中hMLH1,hMSH2及p53的表达进行检测.结果99例子宫内... 董莉,王文革,王莉 - 《实用药物与临床》 被引量: 36发表: 2007年 错配修复基因hMLH1和hMSH2在胃癌中的表达及其临床病理学意义 目的探讨...
Here, reverse phase protein array (RPPA) analysis of MSI2-depleted versus control KrasLA1/+;P53R172H螖G/+ murine NSCLC cell lines identified a significant upregulation of HER3 (ERBB3) upon MSI2 depletion. Negative MSI2-dependent regulation of ERBB3 protein was confirmed in multiple murine ...
In addition, down regulation of MSI2 led to an upregulation of cleaved caspase3 (1.3 fold change;P= 0.029), p27kip1 (1.10 fold change; P= 0.0026) and phospho p53 (1.16 fold change; P= 0.017) compared with siRNA control (siCTR). Collectively, the results highlight the importance of ...
P53 proteinCELL growthB cellsTo identify drivers of sensitivity and resistance to Protein Arginine Methyltransferase 5 (PRMT5) inhibition, we perform a genome-wide CRISPR/Cas9 screen. We identifyand RNA-binding protein() as the top-ranked sensitizer and driver of resistance to specific PRMT5i, ...