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1). To test for an effect of the proline-rich domain on activity, a point mutation was introduced to eliminate the last 39 residues of human POLN by changing Trp codon 861 (TGG) to a stop codon (TGA). POLN and the mutant forms were expressed as NH2-terminal GFP tagged proteins by ...
Replicate was defined as an experimental unit for the trial. Tukey's multiple range test was used to compare the means among treatments. The level of significance was p < 0.05 in all analyses. Results are presented as means and standard error of means. 3. Results 3.1. Growth Performance ...