scRNA-seq has enabled unprecedented immunological discoveries over recent years. To capture the full diversity of the immune system, a large number of cells must be sequenced, which substantially i...
The multiplex scRNA-seq approach is a robust and scalable approach to elucidate lineage and cellular specification across various biological systems.doi:10.1016/j.stemcr.2021.02.006Joachim LuginbühlTsukasa KounoRei NakanoThomas E. ChaterJay W. Shin...
e, scRNA-seq saturation curves for the ECCITE-seq versus standard 10x V(D)J run using the K562 cells expressing the targeting sgRNA library. The same cell suspension was used into two parallel experiments; one underwent a standard 10x V(D)J run while the other was preceded by staining ...
et al. Integrating microarray-based spatial transcriptomics and single-cell RNA-seq reveals tissue architecture in pancreatic ductal adenocarcinomas. Nat. Biotechnol. 38, 333–342 (2020). Among the first to use scRNA-seq data as a reference to resolve cell type information in each microarray-based...
Also, as with the low-throughput application of CRISPRi to repress enhancers, Mosaic-seq cannot distinguish primary enhancer targets from downstream secondary effects. Due to the detection limits of scRNA-seq, applying Mosaic-seq to enhancers that regulate genes with low expression will likely ...
Single-cell RNA sequencing (scRNA-seq) provides high-dimensional measurements of transcript counts in individual cells. However, high assay costs and artifacts associated with analyzing samples across multiple sequencing runs limit the study of large numbers of samples. Sample multiplexing technologies such...
Recommended Applications: Large scale full length viral surveillance Synthetic Construct Sequencing (Amplicons, Plasmids, BACs, etc) Microbiome screening Microbial whole genome sequencing scRNA-seq Low-depth whole genome/GBSplexWell™ Library Prep Workflow: plexWell™ Library Prep Chemistry:Specifications...
Furthermore, with multiplexed scRNA-seq and bulk RNA-seq, we demonstrate the usefulness and necessity of a pooled design to reveal donor iPSC line heterogeneity during macrophage cell differentiation and to model rare WT1 mutation-driven kidney disease with chimeric organoids. Our work provides an ...
Analyzing the high-content readout of scRNA-seq reveals both shared and context-specific transcriptional response components that can identify drug mechanism of action and enable prediction of long-term cell viability from short-term transcriptional responses to treatment....
We validated this with 20 patient-derived models and identified, by 3'end single-cell RNA-sequencing (scRNA-seq) coupled with deconvolution analysis, in vivo models capable of infiltrating along the periphery of either neurons or blood vessels. We previously found that the diverse malignant cells ...