multiplex PCRcytoplasmic male sterilityCommercial exploitation of rice hybrid for sustainable production and productivity largely depends on genetic purity of hybrid seed used. To detect genetic impurity in the hybrid seeds developed through the three-line system using wild abortive (WA) cytoplasmic-based...
Simultaneously, the HPV-positive samples were subjected to a control experiment using the QPCR typing detection method (Shuo Shi Bio HPV nucleic acid typing detection kit—Fluorescent PCR method). The QPCR reaction program included enzyme treatment at 50 °C for 5 min, pre-denaturation at 95 °...
Genetic testing of Mucopolysaccharidoses disease using multiplex PCR- based panels of STR markers: in silico analysis of novel mutationsGenetic testing of Mucopolysaccharidoses disease using multiplex PCR- based panels of STR markers: in silico analysis of novel mutationsMucopolysaccharidoses (MPS)...
One major challenge in the design of highly multiplexed PCR primer sets is the large number of potential primer dimer species that grows quadratically with the number of primers to be designed. Simultaneously, there are exponentially many choices for multiplex primer sequence selection, resulting in...
This invention provides a new and more sensitive methodology for multiplex real time PCR testing of any genetic materials. The method is best illustrated here with the detection and diagnosis of Severe Acute Respiratory Syndrome (SARS) but can be applicable to any other usage where more sensitivity...
2.3.5. AmpliSens® PCR Kit For the PCR amplification of T. vaginalis and M. genitalium, each PCR kit (AmpliSens® Trichomonas vaginalis-EPH PCR Kit and AmpliSens® Mycoplasma genitalium-EPH PCR Kit; InterLabService Ltd, Moscow, Russia) was used in accordance with the manufacturer's prot...
Features Workflow Education Contact Us Instructions for Use In response to the novel coronavirus (SARS-CoV-2) outbreak, Thermo Fisher Scientific has worked rapidly to develop a multiplex real-time PCR diagnostic kit to enable clinical and public health laboratories to quickly diagnose COVID...
6c). To detect deletions within the 7 sgMusk loci, PCR products derived from Musk-cDNA were sequenced and mapped to the genome. In control mice, read junctions (gray) of the PCR products always mapped to individual exons and included the three annotated and the one non-annotated splice ...
For cDNA synthesis, the purified RNA (1 μg) was used in conjunction with PrimeScriptRT Master Mix (TaKaRa, Japan) and diluted 1:20 (volume ratio) for quantitative real-time PCR (qRT-PCR). The QuantStudio3 real-time PCR system (Applied Biosystems, USA) was used for qRT-PCR with the ...
accumulate in diagnostic laboratories. Real-time PCR is also very specific. This specificity, however, is both its strength and its weakness: although the potential for false-positive signals is low, so is the utility of the method for screening to detect related, but not identical, genetic ...