(F) Western blots of IKZF1, IKFZ3, IRF4, CSN5 expression levels after treatment with the indicated dose of LEN in FLAG-CSN5- or empty vector-overexpressing RPMI8226 cells. β-Αctin was used as the loading control. The protein bands in western blotting of IKZF1, IKZF3 and IRF4 wer...
However, only few of them were reported to be transcribed and none were reported to be translated in stem cells. Western blot analysis of OCT4 protein using ESC-like cells revealed multiple bands, indicating that some of the hypothetical pseudogenes are being translated. These novel pseudogenes ...
pallidum surface. Western blot analysis of total proteins of T. pallidum Nichols strain resolved by SDS-PAGE using mouse anti-TP0435 antibodies consist- ently showed the presence of at least two bands even though only the smallest protein is predominantly detected (Fig. 5a). TP0435 ...
The Western Blot was used to measure the TLR2, TLR4, PAD4, and C5ar1 levels in neutrophils (Fig. 8a). Semi-quantitative analysis of the protein bands revealed the TLR2, TLR4, PAD4, and C5ar1 levels were significantly increased in CLP rats with the sham rats as control (P < 0.05 ...
D Levels of DNMT3B mRNA in transfected cells, normalized to GAPDH and expressed as fold change relative to the average value for untransfected cells (real-time PCR and 2−ΔΔCt method). E Representative western blot and densitometric analysis of DNMT3B in transfected cells. Values were ...
The signal intensity of the bands in Western blot membranes or Urea-PAGE gels used in CDA assays was quantified using ImageJ version 1.51d (https://imagej.nih.gov/ij/). P values were calculated by paired T test, and p < 0.05 was defined as significant difference.Abbreviations...
Western blot Whole striata dissected from EAE mice (i.p. treatment starting from 0 dpi) in the acute phase of the disease (24 dpi) were homogenized in RIPA buffer plus protease inhibitor mixture (Sigma) and sonicated. After sonication, the homogenates were centrifuged at 13,000 ×g for 20...
In this study, we aim to investigate the functional roles of bone marrow mesenchymal stem cells (BMSC)-derived exosomal miR-182 on the carfilzomib resistance of MM and its underlying mechanism. Methods qRT-PCR and Western blot methods were utilized to confirm the gene or protein expressions. ...
The average density of the bands was analyzed by Quantity One 4.6.2 software (Bio-Rad Laboratories). The western blot experiments were repeated three times. Literature review We searched for articles in the PubMed (http://www.ncbi.nlm.nih.gov/ pubmed) and WangFang Databases (http://wang...
All bands were normalized to background and mTOR bands were compared with α-tubulin levels. RNA isolation and reverse transcription Total RNA was extracted from flash-frozen P21 mouse sciatic nerves (N = 3 DhhCre(−);Fbxw7fl/fl littermate controls and N = 3 DhhCre(+);Fbxw7...