Analysis of the individual and concatenated gene sequences demonstrated that the nine new strains constituted three stable, well-supported (bootstrap and gene sequence similarity values) monophyletic clusters, A, B and C, all belonging to the branch of the genus Ensifer, regardless of the ...
Sequence and phylogenetic analyses of the amplified 16S rRNA, secA, tuf and SAP11 genes revealed that the detected phytoplasma is a member of the peanut witches'-broom phytoplasma group or 16SrII group ('Candidatus Phytoplasma australasia'). Also, on the basis of computer-simulated RFLP (= in...
DNA sequence analysis of the HXK1 gene, encoding the enzyme GlcNAc-kinase, demonstrated the existence of a specific mutation [guan- ine insertion] that impairs GlcNAc assimilation in C. af- ricana [19]. All of our C. africana carried a specific HXK1 gene mutation and were mating-type a/...
Omar, Ayman F.Sidaros, Samir A.Elsharkawy, Mohsen M.Foissac, XavierSpringer NetherlandsEuropean Journal of Plant PathologyEl-Sisi Y, Omar AF, Sidaros SA, Elsharkawy MM, Foissac X. 2018. Multilo- cus sequence analysis supports a low genetic diversity among `Candi- datus Phytoplasma ...
3.2. Multilocus sequence type determination and comparative analysis of sequence types Currently, a total of 43 STs (housekeeping gene sequence types; HSTs) for seven housekeeping genes were reported in Bordetella MLST database (http://pubmlst.org). These HSTs were established from analysis of coll...
Taxonomic evaluation of the genus Enterobacter based on multilocus sequence analysis (MLSA): proposal to reclassify E. nimipressuralis and E. amnigenus into Lelliottia gen. no. as Lelliottia nimipressuralis comb. nov. and Lelliottia amnigena comb. nov., respectively, E. gergoviae and E....
Multilocus sequence typing revealed that these Borrelia isolates formed a monophyletic group with B. yangtze strains in China. Some of the strains were isolated from the bladders of small mammals, and also two strains were experimentally confirmed to be infectious in C3H/HeN mice. We observed ...
In order to compare experiments it is required that DNA sequencing and analysis is performed using a portable standard. One such standard is multilocus sequence typing (MLST) which was first proposed by Maiden et al. [Maiden et al., 1998]. Currently, MLST is used to type isolates ...
(2004). Discovery of single nucleotide polymorphisms in Lycopersicon esculentum by computer aided analysis of expressed sequence tags. Mol Breed 14: 21–34. Yates HE, Frary A, Doganlar S, Frampton A, Eannetta NT, Uhlig J et al. (2004). Comparative fine mapping of fruit quality QTLs on ...
Further, the MLST data can be subjected to analysis using the eBURST (Based Upon Related Sequence Types) program (https://www.mlst.net/eburst/) to define the relatedness between organisms within a population (due to common ancestry) and to define clonal complexes (CCs). Bastardo and ...