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Briefly, a gradient dilution was performed for IR-26, CO-1080, and HSA@CO-1080 in different solvents, ensuring that the OD at 1064 nm was less than 0.1. The corresponding fluorescence intensity was measured under excitation at 1064 nm (> 1100 nm). The integrated fluorescence intensity...
(3) reversible switching between the dark and green fluorescence states; (4) fluorescence recovery by binding a natural metabolite (i.e. BR) that can be exogenously supplemented when necessary; (5) little fluorescent background under Epi illumination at micromolar ligand concentration that acts as ...
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The BAA fluorophores were purified using high performance liquid chromatography (Agilent 1250 Infinity HPLC) with a C18 column (150 × 21.2 mm) using solvents A: water-formic acid (CH2O2): 99.9:0.1 and B: ACN-CH2O2: 99.9:0.1, with the gradient 90% A to 50% A (6 min) ...