The AmoyDx Pan Lung Cancer PCR Panel (AmoyDx), a multi-gene PCR panel that amplified mutation or fusion variants of 11 genes (EGFR, ALK, ROS1, RET, MET HER2, BRAF, KRAS, and NTRK1-3) by real-time PCR, can be performed with smaller amounts of nucleic acid than NGS, and CS may ...
Profiling the immune repertoire through NGS allows detailed, sequence-specific insight into the adaptive immune response. One of the key challenges during immune receptor amplification is the formation of dimers, which can compete with the immune amplicons of interest during library preparation. Therefore...
Introduction: SLIMamp technology allows multiplex-PCR of tiled amplicons in a single tube, which enables targeting of large exons for NGS analysis with a streamlined process. Copy number variants (CNVs) are DNA segments that are present with variation in the number of copies compared to a normal...
MRD assessment using NGS-based approaches has clear prognostic value and better sensitivity compared to traditional methods. Keywords: multiple myeloma; minimal residual disease; allele-specific oligonucleotide-PCR; droplet digital PCR; next-generation sequencing 1. Introduction The complete response (CR) ...
Correlation of NGS KRAS mutant allele frequency with digital PCR KRAS mutant allele frequency detected in the appropriate multiplex assay for FFPE tissue DNA and cell line gDNA samples.Alexandra, PenderIsaac, Garci...
Digital droplet PCR (ddPCR), as a third-generation PCR technique, offers greater objectivity through absolute quantification and is more cost-effective than NGS, thus assuming a more prominent role in MRD monitoring. Therefore, it is necessary to investigate the utility of detecting mutated gene ...