m Transwell assay to measure modifications in cell migration and invasion capabilities (one-way ANOVA, *P < 0.05, **P < 0.01, ***P < 0.001). Scale bar: 300 μm (f, l); 200 μm (g, m) Full size image To comprehensively elucidate the role of lactylation in OSCC, ...
For hierarchical clustering of genes, dissTOM was employed as a distance measure. The minimum module size (i.e., number of genes) was set to 30, with a merge cut height of 0.25 to detect modules. A total of 16,317 genes (with a coefficient of variation of average RPM (CV > 1...
To measure the diameter of CNT, more than 50 individual CNTs were measured by ImageJ software. The surface chemistry was determined by Fourier-transform infrared spectroscopy (FTIR, Spectrum One, PerkinElmer Management Co., Ltd, Waltham, USA). The gelation degree (G) was calculated using the ...
For specimens embedded in a matrix such as Matrigel, we developed chambers with a straight bottom (Fig. 1f) with a small width to minimize degradation of image quality caused by Matrigel. For samples grown in suspension such as Hydra or gastruloids, we designed chambers with pocket sizes ...
The in vitro unconstrained Achilles tendon is nearly straight, while in vivo experiments reveal that the proximal region of the Achilles tendon, adjacent to Kager’s fat pad, bends ventrally during plantarflexion but remains nearly straight during dorsif
As our MERFISH experiments only measure the expression of ~300 genes, we further assessed its ability to detect cell subsets without matching sc/snRNA-seq data. We compared clustering-based cell annotations obtained from segmented MERFISH to those from RCTD and TACCO-OT (Extended Data Fig. 8d,e...
{0})\)is the perturbation to the liquid-air interface caused by the internal flow. To evaluate the magnitude ofKin the initial stage (regime I), we indirectly measure the interfacial tensions around the three-phase contact line and the droplet edge (method is described in Supplementary ...
(EPL), largely containing straight dendrites and sparse cell bodies, followed by a monolayer of large somata of projection neurons (mitral cell layer) and a densely populated granular inner layer. Furthermore, the OB is a superficial structure, accessible by in vivo functional imaging and it ...
(shown in Fig.3D,F), presynaptic puncta that moved were estimated to be mobile for less than 10% of the time (P13-15: 7.98%; P16-18: 9.93%; P19-21: 4.14%). The same puncta were tracked for each measure of mobility. Since instantaneous velocities were unchanged while distances ...
c Scheme of the TIRF experiment to measure intra-array smFRET. d Microscopic images showing FRET data of single chromatin arrays at 4 mM Mg2+, scale bar: 5 µm. e Trace from dynamic compaction of chromatin fibers by influx of 4 mM Mg2+. f DA1 chromatin fibers compact ...